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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella agona UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP477306SWK
CSB-EP477306SWK
CSB-BP477306SWK
CSB-MP477306SWK
CSB-EP477306SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Pantothenate synthetase (panC) CSB-YP477307SWK
CSB-EP477307SWK
CSB-BP477307SWK
CSB-MP477307SWK
CSB-EP477307SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0229 protein yeaH (yeaH) CSB-YP477308SWK
CSB-EP477308SWK
CSB-BP477308SWK
CSB-MP477308SWK
CSB-EP477308SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L22 (rplV) CSB-YP477309SWK
CSB-EP477309SWK
CSB-BP477309SWK
CSB-MP477309SWK
CSB-EP477309SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L4 (rplD) CSB-YP477310SWK
CSB-EP477310SWK
CSB-BP477310SWK
CSB-MP477310SWK
CSB-EP477310SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Protein TusB (tusB) CSB-YP477311SWK
CSB-EP477311SWK
CSB-BP477311SWK
CSB-MP477311SWK
CSB-EP477311SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Protein slyX (slyX) CSB-YP477312SWK
CSB-EP477312SWK
CSB-BP477312SWK
CSB-MP477312SWK
CSB-EP477312SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0270 protein YheU (yheU) CSB-YP477313SWK
CSB-EP477313SWK
CSB-BP477313SWK
CSB-MP477313SWK
CSB-EP477313SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Fe/S biogenesis protein NfuA (nfuA) CSB-YP477314SWK
CSB-EP477314SWK
CSB-BP477314SWK
CSB-MP477314SWK
CSB-EP477314SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Thiosulfate sulfurtransferase glpE (glpE) CSB-YP477315SWK
CSB-EP477315SWK
CSB-BP477315SWK
CSB-MP477315SWK
CSB-EP477315SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP477316SWK
CSB-EP477316SWK
CSB-BP477316SWK
CSB-MP477316SWK
CSB-EP477316SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Flagellar P-ring protein (flgI) CSB-YP477317SWK
CSB-EP477317SWK
CSB-BP477317SWK
CSB-MP477317SWK
CSB-EP477317SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Multidrug resistance protein MdtG (mdtG), partial CSB-YP477318SWK
CSB-EP477318SWK
CSB-BP477318SWK
CSB-MP477318SWK
CSB-EP477318SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP477319SWK
CSB-EP477319SWK
CSB-BP477319SWK
CSB-MP477319SWK
CSB-EP477319SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP477320VFC
CSB-EP477320VFC
CSB-BP477320VFC
CSB-MP477320VFC
CSB-EP477320VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Probable Fe (2+)-trafficking protein CSB-YP477321VFC
CSB-EP477321VFC
CSB-BP477321VFC
CSB-MP477321VFC
CSB-EP477321VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphoglycerate kinase (pgk) CSB-YP477322VFC
CSB-EP477322VFC
CSB-BP477322VFC
CSB-MP477322VFC
CSB-EP477322VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP477323VFC
CSB-EP477323VFC
CSB-BP477323VFC
CSB-MP477323VFC
CSB-EP477323VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribosome maturation factor RimP (rimP) CSB-YP477324VFC
CSB-EP477324VFC
CSB-BP477324VFC
CSB-MP477324VFC
CSB-EP477324VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri DNA mismatch repair protein MutS (mutS), partial CSB-YP477325VFC
CSB-EP477325VFC
CSB-BP477325VFC
CSB-MP477325VFC
CSB-EP477325VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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