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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella enteritidis PT4 Trp operon repressor (trpR) CSB-YP477526SWO
CSB-EP477526SWO
CSB-BP477526SWO
CSB-MP477526SWO
CSB-EP477526SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 H (+)/Cl (-) exchange transporter ClcA, partial CSB-YP477527SWO
CSB-EP477527SWO
CSB-BP477527SWO
CSB-MP477527SWO
CSB-EP477527SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 [Protein-PII] uridylyltransferase (glnD), partial CSB-YP477528SWO
CSB-EP477528SWO
CSB-BP477528SWO
CSB-MP477528SWO
CSB-EP477528SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Glutamyl-tRNA reductase (hemA) CSB-YP477529SWO
CSB-EP477529SWO
CSB-BP477529SWO
CSB-MP477529SWO
CSB-EP477529SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0115 protein yfcN (yfcN) CSB-YP477530SWO
CSB-EP477530SWO
CSB-BP477530SWO
CSB-MP477530SWO
CSB-EP477530SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Cell division protein ZipA homolog (zipA) CSB-YP477531SWO
CSB-EP477531SWO
CSB-BP477531SWO
CSB-MP477531SWO
CSB-EP477531SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP477532SWO
CSB-EP477532SWO
CSB-BP477532SWO
CSB-MP477532SWO
CSB-EP477532SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Ribonuclease H (rnhA) CSB-YP477533SWO
CSB-EP477533SWO
CSB-BP477533SWO
CSB-MP477533SWO
CSB-EP477533SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP477534SWO
CSB-EP477534SWO
CSB-BP477534SWO
CSB-MP477534SWO
CSB-EP477534SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Uracil phosphoribosyltransferase (upp) CSB-YP477535SWO
CSB-EP477535SWO
CSB-BP477535SWO
CSB-MP477535SWO
CSB-EP477535SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP477536SWO
CSB-EP477536SWO
CSB-BP477536SWO
CSB-MP477536SWO
CSB-EP477536SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 HTH-type transcriptional regulator iscR (iscR) CSB-YP477537SWO
CSB-EP477537SWO
CSB-BP477537SWO
CSB-MP477537SWO
CSB-EP477537SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 50S ribosomal protein L33 (rpmG) CSB-YP477538SWO
CSB-EP477538SWO
CSB-BP477538SWO
CSB-MP477538SWO
CSB-EP477538SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 DNA ligase B (ligB), partial CSB-YP477539SWO
CSB-EP477539SWO
CSB-BP477539SWO
CSB-MP477539SWO
CSB-EP477539SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Esterase FrsA (frsA) CSB-YP477540SWP
CSB-EP477540SWP
CSB-BP477540SWP
CSB-MP477540SWP
CSB-EP477540SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0133 protein ybaB (ybaB) CSB-YP477541SWP
CSB-EP477541SWP
CSB-BP477541SWP
CSB-MP477541SWP
CSB-EP477541SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0271 protein YbgL (ybgL) CSB-YP477542SWP
CSB-EP477542SWP
CSB-BP477542SWP
CSB-MP477542SWP
CSB-EP477542SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0319 protein YccT (yccT) CSB-YP477543SWP
CSB-EP477543SWP
CSB-BP477543SWP
CSB-MP477543SWP
CSB-EP477543SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0259 membrane protein yciC (yciC) CSB-YP477544SWP
CSB-EP477544SWP
CSB-BP477544SWP
CSB-MP477544SWP
CSB-EP477544SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Transcriptional repressor NrdR (nrdR) CSB-YP477545SWP
CSB-EP477545SWP
CSB-BP477545SWP
CSB-MP477545SWP
CSB-EP477545SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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