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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella gallinarum L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP477606SWP
CSB-EP477606SWP
CSB-BP477606SWP
CSB-MP477606SWP
CSB-EP477606SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum C4-dicarboxylate transport protein (dctA), partial CSB-YP477607SWP
CSB-EP477607SWP
CSB-BP477607SWP
CSB-MP477607SWP
CSB-EP477607SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0270 protein YheU (yheU) CSB-YP477608SWP
CSB-EP477608SWP
CSB-BP477608SWP
CSB-MP477608SWP
CSB-EP477608SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 30S ribosomal protein S12 (rpsL) CSB-YP477609SWP
CSB-EP477609SWP
CSB-BP477609SWP
CSB-MP477609SWP
CSB-EP477609SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L3 (rplC) CSB-YP477610SWP
CSB-EP477610SWP
CSB-BP477610SWP
CSB-MP477610SWP
CSB-EP477610SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L16 (rplP) CSB-YP477611SWP
CSB-EP477611SWP
CSB-BP477611SWP
CSB-MP477611SWP
CSB-EP477611SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L5 (rplE) CSB-YP477612SWP
CSB-EP477612SWP
CSB-BP477612SWP
CSB-MP477612SWP
CSB-EP477612SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP477613SWP
CSB-EP477613SWP
CSB-BP477613SWP
CSB-MP477613SWP
CSB-EP477613SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP477614SWP
CSB-EP477614SWP
CSB-BP477614SWP
CSB-MP477614SWP
CSB-EP477614SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 30S ribosomal protein S2 (rpsB) CSB-YP477615SWP
CSB-EP477615SWP
CSB-BP477615SWP
CSB-MP477615SWP
CSB-EP477615SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP477616SWP
CSB-EP477616SWP
CSB-BP477616SWP
CSB-MP477616SWP
CSB-EP477616SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Probable rRNA maturation factor (BDU_64) CSB-YP477617BUH
CSB-EP477617BUH
CSB-BP477617BUH
CSB-MP477617BUH
CSB-EP477617BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L35 (rpmI) CSB-YP477618BUH
CSB-EP477618BUH
CSB-BP477618BUH
CSB-MP477618BUH
CSB-EP477618BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Elongation factor P (efp) CSB-YP477619BUH
CSB-EP477619BUH
CSB-BP477619BUH
CSB-MP477619BUH
CSB-EP477619BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Undecaprenyl-diphosphatase (uppP), partial CSB-YP477620BUH
CSB-EP477620BUH
CSB-BP477620BUH
CSB-MP477620BUH
CSB-EP477620BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 30S ribosomal protein S2 (rpsB) CSB-YP477621BUH
CSB-EP477621BUH
CSB-BP477621BUH
CSB-MP477621BUH
CSB-EP477621BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Glutamate--tRNA ligase (gltX), partial CSB-YP477622BUH
CSB-EP477622BUH
CSB-BP477622BUH
CSB-MP477622BUH
CSB-EP477622BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP477623BUH
CSB-EP477623BUH
CSB-BP477623BUH
CSB-MP477623BUH
CSB-EP477623BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii 50S ribosomal protein L22 (rplV) CSB-YP477624BUH
CSB-EP477624BUH
CSB-BP477624BUH
CSB-MP477624BUH
CSB-EP477624BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Chaperone protein DnaK (dnaK), partial CSB-YP477625BUH
CSB-EP477625BUH
CSB-BP477625BUH
CSB-MP477625BUH
CSB-EP477625BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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