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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Thermosipho africanus Carbon storage regulator homolog (csrA) CSB-YP478759TNG
CSB-EP478759TNG
CSB-BP478759TNG
CSB-MP478759TNG
CSB-EP478759TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus UPF0173 metal-dependent hydrolase THA_544 (THA_544) CSB-YP478760TNG
CSB-EP478760TNG
CSB-BP478760TNG
CSB-MP478760TNG
CSB-EP478760TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L11 (rplK) CSB-YP478761TNG
CSB-EP478761TNG
CSB-BP478761TNG
CSB-MP478761TNG
CSB-EP478761TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus D-alanine--D-alanine ligase (ddl) CSB-YP478762TNG
CSB-EP478762TNG
CSB-BP478762TNG
CSB-MP478762TNG
CSB-EP478762TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP478763TNG
CSB-EP478763TNG
CSB-BP478763TNG
CSB-MP478763TNG
CSB-EP478763TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S12 (rpsL) CSB-YP478764TNG
CSB-EP478764TNG
CSB-BP478764TNG
CSB-MP478764TNG
CSB-EP478764TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L4 (rplD) CSB-YP478765TNG
CSB-EP478765TNG
CSB-BP478765TNG
CSB-MP478765TNG
CSB-EP478765TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S17 (rpsQ) CSB-YP478766TNG
CSB-EP478766TNG
CSB-BP478766TNG
CSB-MP478766TNG
CSB-EP478766TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L6 (rplF) CSB-YP478767TNG
CSB-EP478767TNG
CSB-BP478767TNG
CSB-MP478767TNG
CSB-EP478767TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Probable transcriptional regulatory protein THA_1246 (THA_1246) CSB-YP478768TNG
CSB-EP478768TNG
CSB-BP478768TNG
CSB-MP478768TNG
CSB-EP478768TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative metal-dependent hydrolase BCG9842_B2589 (BCG9842_B2589) CSB-YP478769BQP
CSB-EP478769BQP
CSB-BP478769BQP
CSB-MP478769BQP
CSB-EP478769BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP478770BQP
CSB-EP478770BQP
CSB-BP478770BQP
CSB-MP478770BQP
CSB-EP478770BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0758 protein BCG9842_B0662 (BCG9842_B0662) CSB-YP478771BQP
CSB-EP478771BQP
CSB-BP478771BQP
CSB-MP478771BQP
CSB-EP478771BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamate racemase (murI) CSB-YP478772BQP
CSB-EP478772BQP
CSB-BP478772BQP
CSB-MP478772BQP
CSB-EP478772BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L35 (rpmI) CSB-YP478773BQP
CSB-EP478773BQP
CSB-BP478773BQP
CSB-MP478773BQP
CSB-EP478773BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP478774BQP
CSB-EP478774BQP
CSB-BP478774BQP
CSB-MP478774BQP
CSB-EP478774BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Septation ring formation regulator EzrA (ezrA), partial CSB-YP478775BQP
CSB-EP478775BQP
CSB-BP478775BQP
CSB-MP478775BQP
CSB-EP478775BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Large-conductance mechanosensitive channel (mscL), partial CSB-YP478776BQP1
CSB-EP478776BQP1
CSB-BP478776BQP1
CSB-MP478776BQP1
CSB-EP478776BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP478777BQP
CSB-EP478777BQP
CSB-BP478777BQP
CSB-MP478777BQP
CSB-EP478777BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0234 protein BCG9842_B4128 (BCG9842_B4128) CSB-YP478778BQP
CSB-EP478778BQP
CSB-BP478778BQP
CSB-MP478778BQP
CSB-EP478778BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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