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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella dublin Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP480719SWN
CSB-EP480719SWN
CSB-BP480719SWN
CSB-MP480719SWN
CSB-EP480719SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin D-tyrosyl-tRNA (Tyr) deacylase CSB-YP480720SWN
CSB-EP480720SWN
CSB-BP480720SWN
CSB-MP480720SWN
CSB-EP480720SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP480721SWN
CSB-EP480721SWN
CSB-BP480721SWN
CSB-MP480721SWN
CSB-EP480721SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP480722SWN
CSB-EP480722SWN
CSB-BP480722SWN
CSB-MP480722SWN
CSB-EP480722SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP480723SWN
CSB-EP480723SWN
CSB-BP480723SWN
CSB-MP480723SWN
CSB-EP480723SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0207 protein yfbR (yfbR) CSB-YP480724SWN
CSB-EP480724SWN
CSB-BP480724SWN
CSB-MP480724SWN
CSB-EP480724SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin ATP-dependent protease subunit HslV (hslV) CSB-YP480725SWN
CSB-EP480725SWN
CSB-BP480725SWN
CSB-MP480725SWN
CSB-EP480725SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal protein S6 modification protein (rimK) CSB-YP480726SWN
CSB-EP480726SWN
CSB-BP480726SWN
CSB-MP480726SWN
CSB-EP480726SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Uncharacterized MFS-type transporter ycaD (ycaD), partial CSB-YP480727SWN
CSB-EP480727SWN
CSB-BP480727SWN
CSB-MP480727SWN
CSB-EP480727SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP480728SWN
CSB-EP480728SWN
CSB-BP480728SWN
CSB-MP480728SWN
CSB-EP480728SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Glutamate--tRNA ligase (gltX) CSB-YP480729SWN
CSB-EP480729SWN
CSB-BP480729SWN
CSB-MP480729SWN
CSB-EP480729SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0370 protein ypfN (ypfN), partial CSB-YP480730SWN
CSB-EP480730SWN
CSB-BP480730SWN
CSB-MP480730SWN
CSB-EP480730SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin DnaA-homolog protein hda (hda) CSB-YP480731SWN
CSB-EP480731SWN
CSB-BP480731SWN
CSB-MP480731SWN
CSB-EP480731SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Thiamine-phosphate synthase (thiE) CSB-YP480732SWN
CSB-EP480732SWN
CSB-BP480732SWN
CSB-MP480732SWN
CSB-EP480732SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Homoserine O-succinyltransferase (metA) CSB-YP480733SWN
CSB-EP480733SWN
CSB-BP480733SWN
CSB-MP480733SWN
CSB-EP480733SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Dihydroorotate dehydrogenase (quinone) CSB-YP480734SWN
CSB-EP480734SWN
CSB-BP480734SWN
CSB-MP480734SWN
CSB-EP480734SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP480735SWN
CSB-EP480735SWN
CSB-BP480735SWN
CSB-MP480735SWN
CSB-EP480735SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP480736SWN
CSB-EP480736SWN
CSB-BP480736SWN
CSB-MP480736SWN
CSB-EP480736SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin HTH-type transcriptional regulator iscR (iscR) CSB-YP480737SWN
CSB-EP480737SWN
CSB-BP480737SWN
CSB-MP480737SWN
CSB-EP480737SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Divalent-cation tolerance protein CutA (cutA) CSB-YP480738SWN
CSB-EP480738SWN
CSB-BP480738SWN
CSB-MP480738SWN
CSB-EP480738SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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