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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Zinc finger protein 324A (ZNF324) CSB-YP026673HU
CSB-EP026673HU
CSB-BP026673HU
CSB-MP026673HU
CSB-EP026673HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription initiation protein SPT3 homolog (SUPT3H) CSB-YP022956HU
CSB-BP022956HU
CSB-MP022956HU
CSB-EP022956HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Geminin (GMNN) CSB-YP009577HU
CSB-EP009577HU
CSB-BP009577HU
CSB-MP009577HU
CSB-EP009577HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Claudin-11 (CLDN11), partial CSB-YP005492HU1
CSB-EP005492HU1
CSB-BP005492HU1
CSB-MP005492HU1
CSB-EP005492HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L (TAF5L) CSB-YP023093HU
CSB-BP023093HU
CSB-MP023093HU
CSB-EP023093HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Disabled homolog 1 (DAB1) CSB-YP006479HU
CSB-EP006479HU
CSB-BP006479HU
CSB-MP006479HU
CSB-EP006479HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Low-density lipoprotein receptor-related protein 6 (LRP6), partial CSB-YP013102HU
CSB-EP013102HU
CSB-BP013102HU
CSB-MP013102HU
CSB-EP013102HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PR domain zinc finger protein 1 (PRDM1), partial CSB-YP018638HU
CSB-EP018638HU
CSB-BP018638HU
CSB-MP018638HU
CSB-EP018638HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mitochondrial tRNA-specific 2-thiouridylase 1 (TRMU) CSB-YP024545HU
CSB-EP024545HU
CSB-BP024545HU
CSB-MP024545HU
CSB-EP024545HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Small subunit processome component 20 homolog (UTP20), partial CSB-YP025766HU
CSB-EP025766HU
CSB-BP025766HU
CSB-MP025766HU
CSB-EP025766HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Epididymal secretory glutathione peroxidase (GPX5) CSB-YP009870HU
CSB-BP009870HU
CSB-MP009870HU
CSB-EP009870HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gamma (PIK3C2G), partial CSB-YP017996HU
CSB-EP017996HU
CSB-BP017996HU
CSB-MP017996HU
CSB-EP017996HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription elongation factor A protein 3 (TCEA3) CSB-YP023271HU
CSB-EP023271HU
CSB-BP023271HU
CSB-MP023271HU
CSB-EP023271HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human GRB2-related adapter protein 2 (GRAP2) CSB-YP009884HU
CSB-EP009884HU
CSB-BP009884HU
CSB-MP009884HU
CSB-EP009884HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger MYND domain-containing protein 10 (ZMYND10) CSB-YP026511HU
CSB-EP026511HU
CSB-BP026511HU
CSB-MP026511HU
CSB-EP026511HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 189 (ZNF189) CSB-YP026566HU
CSB-EP026566HU
CSB-BP026566HU
CSB-MP026566HU
CSB-EP026566HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Calcium and integrin-binding family member 2 (CIB2) CSB-YP005427HU
CSB-EP005427HU
CSB-BP005427HU
CSB-MP005427HU
CSB-EP005427HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human CAAX prenyl protease 1 homolog (ZMPSTE24), partial CSB-YP026504HU1
CSB-EP026504HU1
CSB-BP026504HU1
CSB-MP026504HU1
CSB-EP026504HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1) CSB-YP010989HU
CSB-EP010989HU
CSB-BP010989HU
CSB-MP010989HU
CSB-EP010989HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Attractin (ATRN), partial CSB-YP002435HU
CSB-EP002435HU
CSB-BP002435HU
CSB-MP002435HU
CSB-EP002435HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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