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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Flavobacterium johnsoniae Uracil-DNA glycosylase (ung) CSB-YP025641FDT
CSB-EP025641FDT
CSB-BP025641FDT
CSB-MP025641FDT
CSB-EP025641FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Phosphatidylethanolamine N-methyltransferase (CHO2), partial CSB-YP017780LLV
CSB-EP017780LLV
CSB-BP017780LLV
CSB-MP017780LLV
CSB-EP017780LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Histidine ammonia-lyase (hutH), partial CSB-YP010123BPE
CSB-EP010123BPE
CSB-BP010123BPE
CSB-MP010123BPE
CSB-EP010123BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Exoribonuclease 2 (rnb), partial CSB-YP007787VEY
CSB-EP007787VEY
CSB-BP007787VEY
CSB-MP007787VEY
CSB-EP007787VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Uracil-DNA glycosylase (ung) CSB-YP025641VEY
CSB-EP025641VEY
CSB-BP025641VEY
CSB-MP025641VEY
CSB-EP025641VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Adenine phosphoribosyltransferase (apt) CSB-YP001954FDT
CSB-EP001954FDT
CSB-BP001954FDT
CSB-MP001954FDT
CSB-EP001954FDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum CTP synthase (pyrG), partial CSB-YP006176PYG
CSB-EP006176PYG
CSB-BP006176PYG
CSB-MP006176PYG
CSB-EP006176PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Argininosuccinate lyase (argH) CSB-YP002213PYG
CSB-EP002213PYG
CSB-BP002213PYG
CSB-MP002213PYG
CSB-EP002213PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Leucine zipper protein 2 (luzp2) CSB-YP013236DIL
CSB-EP013236DIL
CSB-BP013236DIL
CSB-MP013236DIL
CSB-EP013236DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii Mitochondrial intermediate peptidase (OCT1), partial CSB-YP013835MTM
CSB-EP013835MTM
CSB-BP013835MTM
CSB-MP013835MTM
CSB-EP013835MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lodderomyces elongisporus Mitochondrial intermediate peptidase (OCT1), partial CSB-YP013835LLV
CSB-EP013835LLV
CSB-BP013835LLV
CSB-MP013835LLV
CSB-EP013835LLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Uroporphyrinogen decarboxylase (hemE) CSB-YP025678BPE
CSB-EP025678BPE
CSB-BP025678BPE
CSB-MP025678BPE
CSB-EP025678BPE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678DHZ
CSB-EP025678DHZ
CSB-BP025678DHZ
CSB-MP025678DHZ
CSB-EP025678DHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Cytidine deaminase (cdd) CSB-YP004976VEY
CSB-EP004976VEY
CSB-BP004976VEY
CSB-MP004976VEY
CSB-EP004976VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Adenine phosphoribosyltransferase (apt) CSB-YP001954VEY
CSB-EP001954VEY
CSB-BP001954VEY
CSB-MP001954VEY
CSB-EP001954VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Uroporphyrinogen decarboxylase (hemE) CSB-YP025678VEY
CSB-EP025678VEY
CSB-BP025678VEY
CSB-MP025678VEY
CSB-EP025678VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Thiopurine S-methyltransferase (tpm) CSB-YP024110VEY
CSB-EP024110VEY
CSB-BP024110VEY
CSB-MP024110VEY
CSB-EP024110VEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum GTP cyclohydrolase 1 (folE) CSB-YP009317PYG
CSB-EP009317PYG
CSB-BP009317PYG
CSB-MP009317PYG
CSB-EP009317PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Adenine phosphoribosyltransferase (apt) CSB-YP001954PYG
CSB-EP001954PYG
CSB-BP001954PYG
CSB-MP001954PYG
CSB-EP001954PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meyerozyma guilliermondii 3-hydroxyanthranilate 3,4-dioxygenase (BNA1) CSB-YP010112MTM
CSB-EP010112MTM
CSB-BP010112MTM
CSB-MP010112MTM
CSB-EP010112MTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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