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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Adenine phosphoribosyltransferase (apt) CSB-YP001954SUL
CSB-EP001954SUL
CSB-BP001954SUL
CSB-MP001954SUL
CSB-EP001954SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachemys scripta Glucagon (GCG) CSB-YP009315THC
CSB-EP009315THC
CSB-BP009315THC
CSB-MP009315THC
CSB-EP009315THC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus kisutch Insulin (ins) CSB-YP011742OBH
CSB-EP011742OBH
CSB-BP011742OBH
CSB-MP011742OBH
CSB-EP011742OBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lophius piscatorius Insulin (ins) CSB-YP011742LIG
CSB-EP011742LIG
CSB-BP011742LIG
CSB-MP011742LIG
CSB-EP011742LIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O6 Adenine phosphoribosyltransferase (apt) CSB-YP001954EGX
CSB-EP001954EGX
CSB-BP001954EGX
CSB-MP001954EGX
CSB-EP001954EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans 6-phosphogluconolactonase (pgl) CSB-YP017861AYK
CSB-EP017861AYK
CSB-BP017861AYK
CSB-MP017861AYK
CSB-EP017861AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spermidine synthase (speE) CSB-YP022668BRJ
CSB-EP022668BRJ
CSB-BP022668BRJ
CSB-MP022668BRJ
CSB-EP022668BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121HTA
CSB-EP010121HTA
CSB-BP010121HTA
CSB-MP010121HTA
CSB-EP010121HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Phosphoribosylformylglycinamidine synthase (purL), partial CSB-YP017811SXB
CSB-EP017811SXB
CSB-BP017811SXB
CSB-MP017811SXB
CSB-EP017811SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Transketolase (tkt), partial CSB-YP023580MLW
CSB-EP023580MLW
CSB-BP023580MLW
CSB-MP023580MLW
CSB-EP023580MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fuscata fuscata Fibrinogen alpha chain (FGA) CSB-YP008607MMX
CSB-EP008607MMX
CSB-BP008607MMX
CSB-MP008607MMX
CSB-EP008607MMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Fibrinogen alpha chain (FGA) CSB-YP008607ERJ
CSB-EP008607ERJ
CSB-BP008607ERJ
CSB-MP008607ERJ
CSB-EP008607ERJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anser anser anser Insulin (INS) CSB-YP011742AKX
CSB-EP011742AKX
CSB-BP011742AKX
CSB-MP011742AKX
CSB-EP011742AKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phocoenoides dalli dalli Myoglobin (MB) CSB-YP013529PHS
CSB-EP013529PHS
CSB-BP013529PHS
CSB-MP013529PHS
CSB-EP013529PHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Adenine phosphoribosyltransferase (apt) CSB-YP001954SKX
CSB-EP001954SKX
CSB-BP001954SKX
CSB-MP001954SKX
CSB-EP001954SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrolagus colliei Insulin (ins) CSB-YP011742HQY
CSB-EP011742HQY
CSB-BP011742HQY
CSB-MP011742HQY
CSB-EP011742HQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Uracil-DNA glycosylase (61) CSB-YP025641EFO
CSB-EP025641EFO
CSB-BP025641EFO
CSB-MP025641EFO
CSB-EP025641EFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glutaminase (glsA) CSB-YP009528SSQ
CSB-EP009528SSQ
CSB-BP009528SSQ
CSB-MP009528SSQ
CSB-EP009528SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bos indicus Growth hormone receptor (GHR), partial CSB-YP009411BUL1
CSB-EP009411BUL1
CSB-BP009411BUL1
CSB-MP009411BUL1
CSB-EP009411BUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sargocentron punctatissimum Rhodopsin (rho), partial CSB-YP019681SCQ1
CSB-EP019681SCQ1
CSB-BP019681SCQ1
CSB-MP019681SCQ1
CSB-EP019681SCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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