Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana ADP-ribosylation factor 3 (ARF3) CSB-YP001990DOA
CSB-EP001990DOA
CSB-BP001990DOA
CSB-MP001990DOA
CSB-EP001990DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana temporaria Neurotensin CSB-YP016136RBJ
CSB-EP016136RBJ
CSB-BP016136RBJ
CSB-MP016136RBJ
CSB-EP016136RBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Argininosuccinate lyase (ARG7) CSB-YP002213DSJ
CSB-EP002213DSJ
CSB-BP002213DSJ
CSB-MP002213DSJ
CSB-EP002213DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus megaterium Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232BQX
CSB-EP009232BQX
CSB-BP009232BQX
CSB-MP009232BQX
CSB-EP009232BQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia marcescens Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232SYN
CSB-EP009232SYN
CSB-BP009232SYN
CSB-MP009232SYN
CSB-EP009232SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Annexin A7 (nxnA) CSB-YP001848DKK
CSB-EP001848DKK
CSB-BP001848DKK
CSB-MP001848DKK
CSB-EP001848DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aotus trivirgatus Involucrin (IVL), partial CSB-YP011922DNI
CSB-EP011922DNI
CSB-BP011922DNI
CSB-MP011922DNI
CSB-EP011922DNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina Brain-derived neurotrophic factor (BDNF) CSB-YP002655MPJ
CSB-EP002655MPJ
CSB-BP002655MPJ
CSB-MP002655MPJ
CSB-EP002655MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Adenylate kinase 2 (ADK2) CSB-YP001509SVG
CSB-EP001509SVG
CSB-BP001509SVG
CSB-MP001509SVG
CSB-EP001509SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aminomethyltransferase (gcvT) CSB-YP001684ENV
CSB-EP001684ENV
CSB-BP001684ENV
CSB-MP001684ENV
CSB-EP001684ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calpastatin (Cast) CSB-YP004561RA
CSB-EP004561RA
CSB-BP004561RA
CSB-MP004561RA
CSB-EP004561RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Calcium/calmodulin-dependent protein kinase I (CMK1) CSB-YP004462SVG
CSB-EP004462SVG
CSB-BP004462SVG
CSB-MP004462SVG
CSB-EP004462SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenorhabditis elegans Calreticulin (crt-1) CSB-YP004458CXY
CSB-EP004458CXY
CSB-BP004458CXY
CSB-MP004458CXY
CSB-EP004458CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes albopictus Dihydrofolate reductase (DHFR) CSB-YP006847AXR
CSB-EP006847AXR
CSB-BP006847AXR
CSB-MP006847AXR
CSB-EP006847AXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela vison Prolactin (PRL) CSB-YP018724MVE
CSB-EP018724MVE
CSB-BP018724MVE
CSB-MP018724MVE
CSB-EP018724MVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus niveus Phosphoglycerate kinase 1 (PGK1) CSB-YP017856RDC
CSB-EP017856RDC
CSB-BP017856RDC
CSB-MP017856RDC
CSB-EP017856RDC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas denitrificans Uracil-DNA glycosylase (ung) CSB-YP025641PUH
CSB-EP025641PUH
CSB-BP025641PUH
CSB-MP025641PUH
CSB-EP025641PUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Insulin (INS) CSB-YP011742DSU
CSB-EP011742DSU
CSB-BP011742DSU
CSB-MP011742DSU
CSB-EP011742DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana pipiens Rhodopsin (RHO), partial CSB-YP019681RJN1
CSB-EP019681RJN1
CSB-BP019681RJN1
CSB-MP019681RJN1
CSB-EP019681RJN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Galactose-1-phosphate uridylyltransferase (galT) CSB-YP009226HTA
CSB-EP009226HTA
CSB-BP009226HTA
CSB-MP009226HTA
CSB-EP009226HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>