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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe Eukaryotic translation initiation factor 3 subunit I (tif34) CSB-YP007538SXV
CSB-EP007538SXV
CSB-BP007538SXV
CSB-MP007538SXV
CSB-EP007538SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasypus novemcinctus Aquaporin-2 (AQP2), partial CSB-YP001962DIO1
CSB-EP001962DIO1
CSB-BP001962DIO1
CSB-MP001962DIO1
CSB-EP001962DIO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylobates lar Lysozyme C (LYZ) CSB-YP013283HXW
CSB-EP013283HXW
CSB-BP013283HXW
CSB-MP013283HXW
CSB-EP013283HXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus C5a anaphylatoxin chemotactic receptor (C5AR1), partial CSB-YP003996EXP1
CSB-EP003996EXP1
CSB-BP003996EXP1
CSB-MP003996EXP1
CSB-EP003996EXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes fMet-Leu-Phe receptor (FPR1), partial CSB-YP008854EQV1
CSB-EP008854EQV1
CSB-BP008854EQV1
CSB-MP008854EQV1
CSB-EP008854EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saguinus oedipus Lysozyme C (LYZ) CSB-YP013283SWB
CSB-EP013283SWB
CSB-BP013283SWB
CSB-MP013283SWB
CSB-EP013283SWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiri sciureus Lysozyme C (LYZ) CSB-YP013283SWD
CSB-EP013283SWD
CSB-BP013283SWD
CSB-MP013283SWD
CSB-EP013283SWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes vulpes Agouti-signaling protein (ASIP) CSB-YP002212VFW
CSB-EP002212VFW
CSB-BP002212VFW
CSB-MP002212VFW
CSB-EP002212VFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sonic hedgehog protein (shh) CSB-YP021266AIO
CSB-EP021266AIO
CSB-BP021266AIO
CSB-MP021266AIO
CSB-EP021266AIO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Sonic hedgehog protein (shh) CSB-YP021266DQG
CSB-EP021266DQG
CSB-BP021266DQG
CSB-MP021266DQG
CSB-EP021266DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio Proto-oncogene c-Fos (fos) CSB-YP008790EQE
CSB-EP008790EQE
CSB-BP008790EQE
CSB-MP008790EQE
CSB-EP008790EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dicentrarchus labrax Cytochrome P450 1A1 (cyp1a1), partial CSB-YP006395DKC
CSB-EP006395DKC
CSB-BP006395DKC
CSB-MP006395DKC
CSB-EP006395DKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Desert hedgehog protein (dhh) CSB-YP006851DIL
CSB-EP006851DIL
CSB-BP006851DIL
CSB-MP006851DIL
CSB-EP006851DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytochrome P450 26A1 (cyp26a1) CSB-YP006402DIL
CSB-EP006402DIL
CSB-BP006402DIL
CSB-MP006402DIL
CSB-EP006402DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcebus murinus Presenilin-2 (PSEN2), partial CSB-YP018847MFU
CSB-EP018847MFU
CSB-BP018847MFU
CSB-MP018847MFU
CSB-EP018847MFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Serotransferrin (tf), partial CSB-YP023412OFH
CSB-EP023412OFH
CSB-BP023412OFH
CSB-MP023412OFH
CSB-EP023412OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygathrix nemaeus Lysozyme C (LYZ) CSB-YP013283FHO
CSB-EP013283FHO
CSB-BP013283FHO
CSB-MP013283FHO
CSB-EP013283FHO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rasbora paviei Indian hedgehog protein (ihh) CSB-YP011569RBQ
CSB-EP011569RBQ
CSB-BP011569RBQ
CSB-MP011569RBQ
CSB-EP011569RBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cadherin-4 (cdh4), partial CSB-YP005053XBE
CSB-EP005053XBE
CSB-BP005053XBE
CSB-MP005053XBE
CSB-EP005053XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Neurogenic differentiation factor 4 (neurod4) CSB-YP015727XBE
CSB-EP015727XBE
CSB-BP015727XBE
CSB-MP015727XBE
CSB-EP015727XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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