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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Branchiostoma lanceolatum Cytochrome b (MT-CYB), partial CSB-YP015075BVZ
CSB-EP015075BVZ
CSB-BP015075BVZ
CSB-MP015075BVZ
CSB-EP015075BVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agkistrodon contortrix contortrix NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079AET1
CSB-EP015079AET1
CSB-BP015079AET1
CSB-MP015079AET1
CSB-EP015079AET1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bubalus mindorensis Cytochrome b (MT-CYB), partial CSB-YP015075BMW
CSB-EP015075BMW
CSB-BP015075BMW
CSB-MP015075BMW
CSB-EP015075BMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus robustus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076MAK
CSB-EP015076MAK
CSB-BP015076MAK
CSB-MP015076MAK
CSB-EP015076MAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovophis okinavensis NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079OCY1
CSB-EP015079OCY1
CSB-BP015079OCY1
CSB-MP015079OCY1
CSB-EP015079OCY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii ATP synthase subunit a (MT-ATP6), partial CSB-YP015070PYX1
CSB-EP015070PYX1
CSB-BP015070PYX1
CSB-MP015070PYX1
CSB-EP015070PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079VDN1
CSB-EP015079VDN1
CSB-BP015079VDN1
CSB-MP015079VDN1
CSB-EP015079VDN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothriopsis bilineata Cytochrome b (MT-CYB), partial CSB-YP015075BUQ1
CSB-EP015075BUQ1
CSB-BP015075BUQ1
CSB-MP015075BUQ1
CSB-EP015075BUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius blomhoffii Cytochrome b (MT-CYB), partial CSB-YP015075GGN1
CSB-EP015075GGN1
CSB-BP015075GGN1
CSB-MP015075GGN1
CSB-EP015075GGN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum Adenosylhomocysteinase (SAHH) CSB-YP001474MRC
CSB-EP001474MRC
CSB-BP001474MRC
CSB-MP001474MRC
CSB-EP001474MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 1-delta CSB-YP007431EVU
CSB-EP007431EVU
CSB-BP007431EVU
CSB-MP007431EVU
CSB-EP007431EVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Alcohol dehydrogenase class-3 (FDH) CSB-YP001357ZAX
CSB-EP001357ZAX
CSB-BP001357ZAX
CSB-MP001357ZAX
CSB-EP001357ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae Galactokinase (galK) CSB-YP009198AXG
CSB-EP009198AXG
CSB-BP009198AXG
CSB-MP009198AXG
CSB-EP009198AXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP002355FMR
CSB-EP002355FMR
CSB-BP002355FMR
CSB-MP002355FMR
CSB-EP002355FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacterium thermosaccharolyticum Electron transfer flavoprotein subunit beta (etfB) CSB-YP007845TML
CSB-EP007845TML
CSB-BP007845TML
CSB-MP007845TML
CSB-EP007845TML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3), partial CSB-YP011896MRG
CSB-EP011896MRG
CSB-BP011896MRG
CSB-MP011896MRG
CSB-EP011896MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat C5a anaphylatoxin chemotactic receptor (C5ar1), partial CSB-YP003996RA1
CSB-EP003996RA1
CSB-BP003996RA1
CSB-MP003996RA1
CSB-EP003996RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Heart- and neural crest derivatives-expressed protein 1 (Hand1) CSB-YP010125RA
CSB-EP010125RA
CSB-BP010125RA
CSB-MP010125RA
CSB-EP010125RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hapalemur griseus Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073HCF1
CSB-EP015073HCF1
CSB-BP015073HCF1
CSB-MP015073HCF1
CSB-EP015073HCF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varecia variegata Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073VAN1
CSB-EP015073VAN1
CSB-BP015073VAN1
CSB-MP015073VAN1
CSB-EP015073VAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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