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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bovine Enteropeptidase (TMPRSS15), partial CSB-YP018824BO1
CSB-EP018824BO1
CSB-BP018824BO1
CSB-MP018824BO1
CSB-EP018824BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pagrus major Cytochrome P450 1A1 (cyp1a1), partial CSB-YP006395EQR
CSB-EP006395EQR
CSB-BP006395EQR
CSB-MP006395EQR
CSB-EP006395EQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N-acetylneuraminate lyase (nanA) CSB-YP015994SKY
CSB-EP015994SKY
CSB-BP015994SKY
CSB-MP015994SKY
CSB-EP015994SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosome-recycling factor (frr) CSB-YP014941SKY
CSB-EP014941SKY
CSB-BP014941SKY
CSB-MP014941SKY
CSB-EP014941SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus edulis NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP015080MWU1
CSB-EP015080MWU1
CSB-BP015080MWU1
CSB-MP015080MWU1
CSB-EP015080MWU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudotsuga menziesii Histone H4 CSB-YP010429PWM
CSB-EP010429PWM
CSB-BP010429PWM
CSB-MP010429PWM
CSB-EP010429PWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Adenylate kinase isoenzyme 1 (AK1) CSB-YP001508MRG
CSB-EP001508MRG
CSB-BP001508MRG
CSB-MP001508MRG
CSB-EP001508MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Heat shock-related 70 kDa protein 2 (HSPA2) CSB-YP010824MRG
CSB-EP010824MRG
CSB-BP010824MRG
CSB-MP010824MRG
CSB-EP010824MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Fibrinogen-like protein 1 (FGL1) CSB-YP008653MRG
CSB-EP008653MRG
CSB-BP008653MRG
CSB-MP008653MRG
CSB-EP008653MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus cAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) CSB-YP018694MRG
CSB-EP018694MRG
CSB-BP018694MRG
CSB-MP018694MRG
CSB-EP018694MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelops hesternus Osteocalcin (BGLAP) CSB-YP002682CBQ
CSB-EP002682CBQ
CSB-BP002682CBQ
CSB-MP002682CBQ
CSB-EP002682CBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anarhichas lupus Serum amyloid P-component CSB-YP001898AJS
CSB-EP001898AJS
CSB-BP001898AJS
CSB-MP001898AJS
CSB-EP001898AJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bunodosoma caissarum Phospholipase A2 CSB-YP018091BNJ
CSB-EP018091BNJ
CSB-BP018091BNJ
CSB-MP018091BNJ
CSB-EP018091BNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trypanosoma brucei brucei GPI mannosyltransferase 3 (GPI10), partial CSB-YP017967TIY
CSB-EP017967TIY
CSB-BP017967TIY
CSB-MP017967TIY
CSB-EP017967TIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops leucurus Phospholipase A2 CSB-YP018091BIY
CSB-EP018091BIY
CSB-BP018091BIY
CSB-MP018091BIY
CSB-EP018091BIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Adapter molecule crk (crk) CSB-YP005979XBE
CSB-EP005979XBE
CSB-BP005979XBE
CSB-MP005979XBE
CSB-EP005979XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin gamma-2 chain CSB-YP025336EPO
CSB-EP025336EPO
CSB-BP025336EPO
CSB-MP025336EPO
CSB-EP025336EPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi 60S acidic ribosomal protein P2 (rpp-2) CSB-YP020342BWV
CSB-EP020342BWV
CSB-BP020342BWV
CSB-MP020342BWV
CSB-EP020342BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant COP9 signalosome complex subunit 5 (csn-5) CSB-YP005792CXY
CSB-EP005792CXY
CSB-BP005792CXY
CSB-MP005792CXY
CSB-EP005792CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L15 (rpl-15) CSB-YP020144CXY
CSB-EP020144CXY
CSB-BP020144CXY
CSB-MP020144CXY
CSB-EP020144CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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