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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Casein kinase II subunit alpha (CKA1) CSB-YP006072SVG
CSB-EP006072SVG
CSB-BP006072SVG
CSB-MP006072SVG
CSB-EP006072SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio Cytochrome c oxidase subunit 3 (mt-co3), partial CSB-YP015074EQE1
CSB-EP015074EQE1
CSB-BP015074EQE1
CSB-MP015074EQE1
CSB-EP015074EQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina ATP synthase subunit a (ATP6), partial CSB-YP015070EXJ1
CSB-EP015070EXJ1
CSB-BP015070EXJ1
CSB-MP015070EXJ1
CSB-EP015070EXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus ATP synthase protein 8 (MT-ATP8), partial CSB-YP015071FOT
CSB-EP015071FOT
CSB-BP015071FOT
CSB-MP015071FOT
CSB-EP015071FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Estrogen receptor (esr1), partial CSB-YP007830OEI
CSB-EP007830OEI
CSB-BP007830OEI
CSB-MP007830OEI
CSB-EP007830OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum 60S ribosomal protein L11 (rpl11) CSB-YP020115DKK
CSB-EP020115DKK
CSB-BP020115DKK
CSB-MP020115DKK
CSB-EP020115DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thomomys townsendii Cytochrome b (MT-CYB), partial CSB-YP015075TED
CSB-EP015075TED
CSB-BP015075TED
CSB-MP015075TED
CSB-EP015075TED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase II subunit RPB3 (RPB3) CSB-YP018329SVG
CSB-EP018329SVG
CSB-BP018329SVG
CSB-MP018329SVG
CSB-EP018329SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA dimethylallyltransferase (miaA) CSB-YP024529ENV
CSB-EP024529ENV
CSB-BP024529ENV
CSB-MP024529ENV
CSB-EP024529ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit fibroma virus DNA topoisomerase 1 (TOP1) CSB-YP024056RAA
CSB-EP024056RAA
CSB-BP024056RAA
CSB-MP024056RAA
CSB-EP024056RAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Substance-K receptor (Tacr2), partial CSB-YP023069RA1
CSB-EP023069RA1
CSB-BP023069RA1
CSB-MP023069RA1
CSB-EP023069RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gap junction alpha-1 protein (gja1), partial CSB-YP009443XBE1
CSB-EP009443XBE1
CSB-BP009443XBE1
CSB-MP009443XBE1
CSB-EP009443XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max 40S ribosomal protein S11 (RPS11) CSB-YP020365GGV
CSB-EP020365GGV
CSB-BP020365GGV
CSB-MP020365GGV
CSB-EP020365GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Neuromodulin (gap43) CSB-YP009231DQG
CSB-EP009231DQG
CSB-BP009231DQG
CSB-MP009231DQG
CSB-EP009231DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lithobates catesbeiana Parvalbumin alpha CSB-YP019092LQA
CSB-EP019092LQA
CSB-BP019092LQA
CSB-MP019092LQA
CSB-EP019092LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anguilla japonica Natriuretic peptides A (nppa) CSB-YP016020BZH
CSB-EP016020BZH
CSB-BP016020BZH
CSB-MP016020BZH
CSB-EP016020BZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus T-complex protein 1 subunit alpha (TCP1), partial CSB-YP023320DXU
CSB-EP023320DXU
CSB-BP023320DXU
CSB-MP023320DXU
CSB-EP023320DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Tubulin beta chain CSB-YP025318FOT
CSB-EP025318FOT
CSB-BP025318FOT
CSB-MP025318FOT
CSB-EP025318FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster NADH-ubiquinone oxidoreductase chain 3 (mt:ND3), partial CSB-YP015078DLU1
CSB-EP015078DLU1
CSB-BP015078DLU1
CSB-MP015078DLU1
CSB-EP015078DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix argentata Hemoglobin subunit alpha (HBA) CSB-YP010147CBA
CSB-EP010147CBA
CSB-BP010147CBA
CSB-MP010147CBA
CSB-EP010147CBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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