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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (SSU72) CSB-YP022729CTK
CSB-EP022729CTK
CSB-BP022729CTK
CSB-MP022729CTK
CSB-EP022729CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Transcription initiation factor IIA subunit 2 (TOA2) CSB-YP010000CTK
CSB-EP010000CTK
CSB-BP010000CTK
CSB-MP010000CTK
CSB-EP010000CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial import inner membrane translocase subunit TIM13 (TIM13) CSB-YP023549CTL
CSB-EP023549CTL
CSB-BP023549CTL
CSB-MP023549CTL
CSB-EP023549CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Flap endonuclease 1 (FEN1) CSB-YP008585CTK
CSB-EP008585CTK
CSB-BP008585CTK
CSB-MP008585CTK
CSB-EP008585CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cytoplasmic tRNA 2-thiolation protein 1 (NCS6) CSB-YP002426CTL
CSB-EP002426CTL
CSB-BP002426CTL
CSB-MP002426CTL
CSB-EP002426CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 ATP synthase subunit b (atpF), partial CSB-YP002358SMW1
CSB-EP002358SMW1
CSB-BP002358SMW1
CSB-MP002358SMW1
CSB-EP002358SMW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 ATP synthase subunit beta (atpD) CSB-YP002350SMW
CSB-EP002350SMW
CSB-BP002350SMW
CSB-MP002350SMW
CSB-EP002350SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP025342SMV
CSB-EP025342SMV
CSB-BP025342SMV
CSB-MP025342SMV
CSB-EP025342SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 GTPase Era (era) CSB-YP007759SMW
CSB-EP007759SMW
CSB-BP007759SMW
CSB-MP007759SMW
CSB-EP007759SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SMW
CSB-EP015150SMW
CSB-BP015150SMW
CSB-MP015150SMW
CSB-EP015150SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Thymidine kinase (tdk) CSB-YP023577SMW
CSB-EP023577SMW
CSB-BP023577SMW
CSB-MP023577SMW
CSB-EP023577SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Ribose-phosphate pyrophosphokinase 1 (prs1) CSB-YP018776SMW
CSB-EP018776SMW
CSB-BP018776SMW
CSB-MP018776SMW
CSB-EP018776SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Thymidine kinase (Mal-058) CSB-YP023577AYE
CSB-EP023577AYE
CSB-BP023577AYE
CSB-MP023577AYE
CSB-EP023577AYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadrurus gertschi Phospholipase A2 CSB-YP018091HST
CSB-EP018091HST
CSB-BP018091HST
CSB-MP018091HST
CSB-EP018091HST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Draxin CSB-YP003559BO
CSB-EP003559BO
CSB-BP003559BO
CSB-MP003559BO
CSB-EP003559BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (NDUFA8) CSB-YP015633EXP
CSB-EP015633EXP
CSB-BP015633EXP
CSB-MP015633EXP
CSB-EP015633EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (NDUFA5) CSB-YP015629PYX
CSB-EP015629PYX
CSB-BP015629PYX
CSB-MP015629PYX
CSB-EP015629PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Thymidine kinase (Ken-062) CSB-YP023577AEB
CSB-EP023577AEB
CSB-BP023577AEB
CSB-MP023577AEB
CSB-EP023577AEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus biovar 1 Glucokinase (glk) CSB-YP009319BWS
CSB-EP009319BWS
CSB-BP009319BWS
CSB-MP009319BWS
CSB-EP009319BWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (NDUFB1), partial CSB-YP015641EXP
CSB-EP015641EXP
CSB-BP015641EXP
CSB-MP015641EXP
CSB-EP015641EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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