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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Diphthamide biosynthesis protein 1 (DPH1), partial CSB-YP007129CTL
CSB-EP007129CTL
CSB-BP007129CTL
CSB-MP007129CTL
CSB-EP007129CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit B (PRT1), partial CSB-YP007530CTK
CSB-EP007530CTK
CSB-BP007530CTK
CSB-MP007530CTK
CSB-EP007530CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit D (CNB01420), partial CSB-YP007533CTL
CSB-EP007533CTL
CSB-BP007533CTL
CSB-MP007533CTL
CSB-EP007533CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit E (INT6) CSB-YP007534CTL
CSB-EP007534CTL
CSB-BP007534CTL
CSB-MP007534CTL
CSB-EP007534CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit K (CND02370) CSB-YP007540CTL
CSB-EP007540CTL
CSB-BP007540CTL
CSB-MP007540CTL
CSB-EP007540CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Exportin-T (LOS1), partial CSB-YP026226CTL
CSB-EP026226CTL
CSB-BP026226CTL
CSB-MP026226CTL
CSB-EP026226CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial import inner membrane translocase subunit TIM50 (TIM50), partial CSB-YP023556CTK1
CSB-EP023556CTK1
CSB-BP023556CTK1
CSB-MP023556CTK1
CSB-EP023556CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Structure-specific endonuclease subunit SLX1 (SLX1) CSB-YP009442CTK
CSB-EP009442CTK
CSB-BP009442CTK
CSB-MP009442CTK
CSB-EP009442CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Glutamine synthetase (GLN1) CSB-YP009553CTK
CSB-EP009553CTK
CSB-BP009553CTK
CSB-MP009553CTK
CSB-EP009553CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A.Z (HTZ1) CSB-YP010100CTL
CSB-EP010100CTL
CSB-BP010100CTL
CSB-MP010100CTL
CSB-EP010100CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Inositol-pentakisphosphate 2-kinase (IPK1) CSB-YP011783CTK
CSB-EP011783CTK
CSB-BP011783CTK
CSB-MP011783CTK
CSB-EP011783CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynurenine 3-monooxygenase (BNA4), partial CSB-YP012475CTL
CSB-EP012475CTL
CSB-BP012475CTL
CSB-MP012475CTL
CSB-EP012475CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5) CSB-YP012703CTL
CSB-EP012703CTL
CSB-BP012703CTL
CSB-MP012703CTL
CSB-EP012703CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial Rho GTPase 1 (GEM1), partial CSB-YP019695CTK1
CSB-EP019695CTK1
CSB-BP019695CTK1
CSB-MP019695CTK1
CSB-EP019695CTK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit F (CNBF0360) CSB-YP007535CTK
CSB-EP007535CTK
CSB-BP007535CTK
CSB-MP007535CTK
CSB-EP007535CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Myosin-1 (MYO1), partial CSB-YP015289CTK
CSB-EP015289CTK
CSB-BP015289CTK
CSB-MP015289CTK
CSB-EP015289CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Nascent polypeptide-associated complex subunit alpha (EGD2) CSB-YP015403CTL
CSB-EP015403CTL
CSB-BP015403CTL
CSB-MP015403CTL
CSB-EP015403CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Nucleolar protein 16 (NOP16) CSB-YP015937CTK
CSB-EP015937CTK
CSB-BP015937CTK
CSB-MP015937CTK
CSB-EP015937CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D GPI mannosyltransferase 2 (GPI18), partial CSB-YP017985CTK
CSB-EP017985CTK
CSB-BP017985CTK
CSB-MP017985CTK
CSB-EP017985CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Peptidyl-prolyl cis-trans isomerase D (CPR6) CSB-YP018474CTL
CSB-EP018474CTL
CSB-BP018474CTL
CSB-MP018474CTL
CSB-EP018474CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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