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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit L (CNL05160), partial CSB-YP007541CTL
CSB-EP007541CTL
CSB-BP007541CTL
CSB-MP007541CTL
CSB-EP007541CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Structure-specific endonuclease subunit SLX1 (SLX1) CSB-YP009442CTL
CSB-EP009442CTL
CSB-BP009442CTL
CSB-MP009442CTL
CSB-EP009442CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Glutamine synthetase (GLN1) CSB-YP009553CTL
CSB-EP009553CTL
CSB-BP009553CTL
CSB-MP009553CTL
CSB-EP009553CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H2A.Z (HTZ1) CSB-YP010100CTK
CSB-EP010100CTK
CSB-BP010100CTK
CSB-MP010100CTK
CSB-EP010100CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor SYF1 (SYF1), partial CSB-YP026171CTL
CSB-EP026171CTL
CSB-BP026171CTL
CSB-MP026171CTL
CSB-EP026171CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynurenine 3-monooxygenase (BNA4), partial CSB-YP012475CTK
CSB-EP012475CTK
CSB-BP012475CTK
CSB-MP012475CTK
CSB-EP012475CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Kynureninase (BNA5) CSB-YP012703CTK
CSB-EP012703CTK
CSB-BP012703CTK
CSB-MP012703CTK
CSB-EP012703CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D mRNA cap guanine-N7 methyltransferase (ABD1), partial CSB-YP019902CTL
CSB-EP019902CTL
CSB-BP019902CTL
CSB-MP019902CTL
CSB-EP019902CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mediator of RNA polymerase II transcription subunit 4 (MED4) CSB-YP013665CTL
CSB-EP013665CTL
CSB-BP013665CTL
CSB-MP013665CTL
CSB-EP013665CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mediator of RNA polymerase II transcription subunit 7 (MED7) CSB-YP013667CTL
CSB-EP013667CTL
CSB-BP013667CTL
CSB-MP013667CTL
CSB-EP013667CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Nucleolar protein 58 (NOP58), partial CSB-YP015940CTK
CSB-EP015940CTK
CSB-BP015940CTK
CSB-MP015940CTK
CSB-EP015940CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Nuclear distribution protein nudE homolog 1 (NDE1), partial CSB-YP015602CTL
CSB-EP015602CTL
CSB-BP015602CTL
CSB-MP015602CTL
CSB-EP015602CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D GPI mannosyltransferase 2 (GPI18), partial CSB-YP017985CTL
CSB-EP017985CTL
CSB-BP017985CTL
CSB-MP017985CTL
CSB-EP017985CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Peptidyl-prolyl cis-trans isomerase-like 3 (CYP10) CSB-YP018484CTK
CSB-EP018484CTK
CSB-BP018484CTK
CSB-MP018484CTK
CSB-EP018484CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D FACT complex subunit SPT16 (SPT16), partial CSB-YP022955CTL
CSB-EP022955CTL
CSB-BP022955CTL
CSB-MP022955CTL
CSB-EP022955CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D DNA replication complex GINS protein PSF1 (PSF1) CSB-YP009430CTK
CSB-EP009430CTK
CSB-BP009430CTK
CSB-MP009430CTK
CSB-EP009430CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Ribose-5-phosphate isomerase (RKI1) CSB-YP020104CTL
CSB-EP020104CTL
CSB-BP020104CTL
CSB-MP020104CTL
CSB-EP020104CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor SLU7 (SLU7), partial CSB-YP021783CTK
CSB-EP021783CTK
CSB-BP021783CTK
CSB-MP021783CTK
CSB-EP021783CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Transcription elongation factor SPT5 (SPT5), partial CSB-YP022959CTL
CSB-EP022959CTL
CSB-BP022959CTL
CSB-MP022959CTL
CSB-EP022959CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Transcription elongation factor SPT6 (SPT6), partial CSB-YP022960CTL
CSB-EP022960CTL
CSB-BP022960CTL
CSB-MP022960CTL
CSB-EP022960CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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