Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L10 (RPL10) CSB-YP020106SVG
CSB-EP020106SVG
CSB-BP020106SVG
CSB-MP020106SVG
CSB-EP020106SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribonucleases P/MRP protein subunit POP1 (POP1), partial CSB-YP018372SVG
CSB-EP018372SVG
CSB-BP018372SVG
CSB-MP018372SVG
CSB-EP018372SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Low molecular weight phosphotyrosine protein phosphatase (stp1) CSB-YP001176SXV
CSB-EP001176SXV
CSB-BP001176SXV
CSB-MP001176SXV
CSB-EP001176SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine decarboxylase (odc-1) CSB-YP016269CXY
CSB-EP016269CXY
CSB-BP016269CXY
CSB-MP016269CXY
CSB-EP016269CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia pahangi 60S ribosomal protein L15 CSB-YP020144BWW
CSB-EP020144BWW
CSB-BP020144BWW
CSB-MP020144BWW
CSB-EP020144BWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dirofilaria immitis Extracellular superoxide dismutase [Cu-Zn] CSB-YP022399DKS
CSB-EP022399DKS
CSB-BP022399DKS
CSB-MP022399DKS
CSB-EP022399DKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-complex protein 1 subunit alpha (cct-1), partial CSB-YP023320CXY
CSB-EP023320CXY
CSB-BP023320CXY
CSB-MP023320CXY
CSB-EP023320CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant U3 small nucleolar RNA-associated protein 18 homolog (B0280.9) CSB-YP025765CXY
CSB-EP025765CXY
CSB-BP025765CXY
CSB-MP025765CXY
CSB-EP025765CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus ATP synthase subunit alpha (atpA), partial CSB-YP002344GFM
CSB-EP002344GFM
CSB-BP002344GFM
CSB-MP002344GFM
CSB-EP002344GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Davidiella tassiana 60S acidic ribosomal protein P2 (CLAH5) CSB-YP020342DAY
CSB-EP020342DAY
CSB-BP020342DAY
CSB-MP020342DAY
CSB-EP020342DAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alternaria alternata Aldehyde dehydrogenase (ALTA10) CSB-YP001572AZV
CSB-EP001572AZV
CSB-BP001572AZV
CSB-MP001572AZV
CSB-EP001572AZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis L-lactate dehydrogenase B chain (ldhb) CSB-YP012841XBE
CSB-EP012841XBE
CSB-BP012841XBE
CSB-MP012841XBE
CSB-EP012841XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis L-lactate dehydrogenase C chain (ldhc) CSB-YP012844XBE
CSB-EP012844XBE
CSB-BP012844XBE
CSB-MP012844XBE
CSB-EP012844XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat L-lactate dehydrogenase B chain (Ldhb) CSB-YP012841RA
CSB-EP012841RA
CSB-BP012841RA
CSB-MP012841RA
CSB-EP012841RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antechinus stuartii Sperm protamine P1 (PRM1) CSB-YP018728ALC
CSB-EP018728ALC
CSB-BP018728ALC
CSB-MP018728ALC
CSB-EP018728ALC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenolestes fuliginosus Sperm protamine P1 (PRM1) CSB-YP018728CAD
CSB-EP018728CAD
CSB-BP018728CAD
CSB-MP018728CAD
CSB-EP018728CAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isoodon macrourus Sperm protamine P1 (PRM1) CSB-YP018728ILA
CSB-EP018728ILA
CSB-BP018728ILA
CSB-MP018728ILA
CSB-EP018728ILA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus giganteus Sperm protamine P1 (PRM1) CSB-YP018728MPF
CSB-EP018728MPF
CSB-BP018728MPF
CSB-MP018728MPF
CSB-EP018728MPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Notoryctes typhlops Sperm protamine P1 (PRM1) CSB-YP018728NHW
CSB-EP018728NHW
CSB-BP018728NHW
CSB-MP018728NHW
CSB-EP018728NHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perameles gunnii Sperm protamine P1 (PRM1) CSB-YP018728ETQ
CSB-EP018728ETQ
CSB-BP018728ETQ
CSB-MP018728ETQ
CSB-EP018728ETQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>