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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arctocephalus galapagoensis Hemoglobin subunit alpha-2 CSB-YP010148ANR
CSB-EP010148ANR
CSB-BP010148ANR
CSB-MP010148ANR
CSB-EP010148ANR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain (BTU1) CSB-YP025318TLU
CSB-EP025318TLU
CSB-BP025318TLU
CSB-MP025318TLU
CSB-EP025318TLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Tubulin beta chain CSB-YP025318BTT
CSB-EP025318BTT
CSB-BP025318BTT
CSB-MP025318BTT
CSB-EP025318BTT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Amidophosphoribosyltransferase (ade4), partial CSB-YP018427SXV
CSB-EP018427SXV
CSB-BP018427SXV
CSB-MP018427SXV
CSB-EP018427SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA topoisomerase 1 (top1), partial CSB-YP024056XBE
CSB-EP024056XBE
CSB-BP024056XBE
CSB-MP024056XBE
CSB-EP024056XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (OST1), partial CSB-YP020344SVG1
CSB-EP020344SVG1
CSB-BP020344SVG1
CSB-MP020344SVG1
CSB-EP020344SVG1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cat Leucine-rich repeat and calponin homology domain-containing protein 1 CSB-YP013071CA
CSB-EP013071CA
CSB-BP013071CA
CSB-MP013071CA
CSB-EP013071CA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulatus Tubulin beta chain (TUB2) CSB-YP025318AZC
CSB-EP025318AZC
CSB-BP025318AZC
CSB-MP025318AZC
CSB-EP025318AZC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeosphaeria nodorum Tubulin beta chain (tubB) CSB-YP025318EUG
CSB-EP025318EUG
CSB-BP025318EUG
CSB-MP025318EUG
CSB-EP025318EUG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit H (VMA13) CSB-YP002413SVG
CSB-EP002413SVG
CSB-BP002413SVG
CSB-MP002413SVG
CSB-EP002413SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Coatomer subunit beta (SEC26), partial CSB-YP005783SVG
CSB-EP005783SVG
CSB-BP005783SVG
CSB-MP005783SVG
CSB-EP005783SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine herpesvirus 2 Thymidine kinase (TK) CSB-YP023577BJX
CSB-EP023577BJX
CSB-BP023577BJX
CSB-MP023577BJX
CSB-EP023577BJX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UDP-glucose 4-epimerase (galE) CSB-YP009197SXB
CSB-EP009197SXB
CSB-BP009197SXB
CSB-MP009197SXB
CSB-EP009197SXB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteles cristata Hemoglobin subunit beta (HBB) CSB-YP010150PSO
CSB-EP010150PSO
CSB-BP010150PSO
CSB-MP010150PSO
CSB-EP010150PSO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tubulin gamma-1 chain (tubg1) CSB-YP025334XBE
CSB-EP025334XBE
CSB-BP025334XBE
CSB-MP025334XBE
CSB-EP025334XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 40S ribosomal protein S20 (rps20) CSB-YP020394XBE
CSB-EP020394XBE
CSB-BP020394XBE
CSB-MP020394XBE
CSB-EP020394XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Heme oxygenase 2 (Hmox2) CSB-YP010584RA
CSB-EP010584RA
CSB-BP010584RA
CSB-MP010584RA
CSB-EP010584RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis GATA-binding factor 2 (gata2) CSB-YP009275XBE
CSB-EP009275XBE
CSB-BP009275XBE
CSB-MP009275XBE
CSB-EP009275XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mitochondrial-processing peptidase subunit alpha (mpp), partial CSB-YP018242NHA
CSB-EP018242NHA
CSB-BP018242NHA
CSB-MP018242NHA
CSB-EP018242NHA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious laryngotracheitis virus Thymidine kinase (TK) CSB-YP023577IAS
CSB-EP023577IAS
CSB-BP023577IAS
CSB-MP023577IAS
CSB-EP023577IAS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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