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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter capsulatus Hypoxanthine-guanine phosphoribosyltransferase (hpt) CSB-YP010706RLD
CSB-EP010706RLD
CSB-BP010706RLD
CSB-MP010706RLD
CSB-EP010706RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida tropicalis NADPH--cytochrome P450 reductase (NCP1), partial CSB-YP018378CZN1
CSB-EP018378CZN1
CSB-BP018378CZN1
CSB-MP018378CZN1
CSB-EP018378CZN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum NADP-dependent malic enzyme (MOD1), partial CSB-YP013634MRC
CSB-EP013634MRC
CSB-BP013634MRC
CSB-MP013634MRC
CSB-EP013634MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ferrochelatase (hemH) CSB-YP008579SXB
CSB-EP008579SXB
CSB-BP008579SXB
CSB-MP008579SXB
CSB-EP008579SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP synthase subunit delta (atpH) CSB-YP002355BRJ
CSB-EP002355BRJ
CSB-BP002355BRJ
CSB-MP002355BRJ
CSB-EP002355BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pleurodeles waltl Transcription factor SOX-1 (SOX1) CSB-YP022417EWZ
CSB-BP022417EWZ
CSB-MP022417EWZ
CSB-EP022417EWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA-3-methyladenine glycosylase (alkA) CSB-YP014749BRJ
CSB-EP014749BRJ
CSB-BP014749BRJ
CSB-MP014749BRJ
CSB-EP014749BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus G2/mitotic-specific cyclin-B2 (CCNB2) CSB-YP004808MRG
CSB-EP004808MRG
CSB-BP004808MRG
CSB-MP004808MRG
CSB-EP004808MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycerol-3-phosphate transporter (glpT), partial CSB-YP021610BRJ
CSB-EP021610BRJ
CSB-BP021610BRJ
CSB-MP021610BRJ
CSB-EP021610BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida tropicalis V-type proton ATPase catalytic subunit A (VMA1) CSB-YP002396CZN
CSB-EP002396CZN
CSB-BP002396CZN
CSB-MP002396CZN
CSB-EP002396CZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ADP-ribosylation factor-like protein 1 (ARL1) CSB-YP002070SVG
CSB-EP002070SVG
CSB-BP002070SVG
CSB-MP002070SVG
CSB-EP002070SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CHK1 (CHK1), partial CSB-YP005338SVG
CSB-EP005338SVG
CSB-BP005338SVG
CSB-MP005338SVG
CSB-EP005338SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein phosphatase 4 regulatory subunit 2 (PSY4) CSB-YP018580SVG
CSB-EP018580SVG
CSB-BP018580SVG
CSB-MP018580SVG
CSB-EP018580SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GPI mannosyltransferase 2 (GPI18), partial CSB-YP017985SVG
CSB-EP017985SVG
CSB-BP017985SVG
CSB-MP017985SVG
CSB-EP017985SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB2 (RPB135), partial CSB-YP018328DOA
CSB-EP018328DOA
CSB-BP018328DOA
CSB-MP018328DOA
CSB-EP018328DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP-binding nuclear protein Ran CSB-YP019307PLO
CSB-EP019307PLO
CSB-BP019307PLO
CSB-MP019307PLO
CSB-EP019307PLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Inorganic pyrophosphatase (ppa) CSB-YP018410TNT
CSB-EP018410TNT
CSB-BP018410TNT
CSB-MP018410TNT
CSB-EP018410TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus ATP synthase subunit a (MT-ATP6), partial CSB-YP015070HAU1
CSB-EP015070HAU1
CSB-BP015070HAU1
CSB-MP015070HAU1
CSB-EP015070HAU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074HAU1
CSB-EP015074HAU1
CSB-BP015074HAU1
CSB-MP015074HAU1
CSB-EP015074HAU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus NADH-ubiquinone oxidoreductase chain 6 (MT-ND6), partial CSB-YP015082HAU1
CSB-EP015082HAU1
CSB-BP015082HAU1
CSB-MP015082HAU1
CSB-EP015082HAU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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