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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant ATP synthase subunit b (atpF), partial CSB-YP002358RMA1
CSB-EP002358RMA1
CSB-BP002358RMA1
CSB-MP002358RMA1
CSB-EP002358RMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycogen debranching enzyme (glgX) CSB-YP001446ENV
CSB-EP001446ENV
CSB-BP001446ENV
CSB-MP001446ENV
CSB-EP001446ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Elongation factor 2 (efbA), partial CSB-YP007434DKK
CSB-EP007434DKK
CSB-BP007434DKK
CSB-MP007434DKK
CSB-EP007434DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cytochrome P450 4B1 (Cyp4b1) CSB-YP006448RA
CSB-EP006448RA
CSB-BP006448RA
CSB-MP006448RA
CSB-EP006448RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Cytochrome c oxidase subunit 3 (COIII), partial CSB-YP015074FOT1
CSB-EP015074FOT1
CSB-BP015074FOT1
CSB-MP015074FOT1
CSB-EP015074FOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP015080FOT1
CSB-EP015080FOT1
CSB-BP015080FOT1
CSB-MP015080FOT1
CSB-EP015080FOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leishmania tarentolae NADH-ubiquinone oxidoreductase chain 1 (ND1), partial CSB-YP015076LOI
CSB-EP015076LOI
CSB-BP015076LOI
CSB-MP015076LOI
CSB-EP015076LOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b (MT-CYB), partial CSB-YP015075ERW
CSB-EP015075ERW
CSB-BP015075ERW
CSB-MP015075ERW
CSB-EP015075ERW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Paired box protein Pax-1 (PAX1) CSB-YP017487HU
CSB-EP017487HU
CSB-BP017487HU
CSB-MP017487HU
CSB-EP017487HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Histone H1.1 CSB-YP010376TQN
CSB-EP010376TQN
CSB-BP010376TQN
CSB-MP010376TQN
CSB-EP010376TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans NADH-ubiquinone oxidoreductase chain 3 (nd3), partial CSB-YP015078EBP1
CSB-EP015078EBP1
CSB-BP015078EBP1
CSB-MP015078EBP1
CSB-EP015078EBP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Deoxycytidylate deaminase (CD) CSB-YP006563EDZ
CSB-EP006563EDZ
CSB-BP006563EDZ
CSB-MP006563EDZ
CSB-EP006563EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erysiphe graminis subsp. hordei Tubulin beta chain (TUB2) CSB-YP025318END
CSB-EP025318END
CSB-BP025318END
CSB-MP025318END
CSB-EP025318END-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Guanylate cyclase soluble subunit beta-1 (GUCY1B1), partial CSB-YP010052BO
CSB-EP010052BO
CSB-BP010052BO
CSB-MP010052BO
CSB-EP010052BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078ZAX1
CSB-EP015078ZAX1
CSB-BP015078ZAX1
CSB-MP015078ZAX1
CSB-EP015078ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Platelet endothelial cell adhesion molecule (PECAM1), partial CSB-YP017767HU1
CSB-EP017767HU1
CSB-BP017767HU1
CSB-MP017767HU1
CSB-EP017767HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pomatostomus isidorei Cytochrome b (MT-CYB), partial CSB-YP015075PPJ
CSB-EP015075PPJ
CSB-BP015075PPJ
CSB-MP015075PPJ
CSB-EP015075PPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1), partial CSB-YP001593SVG1
CSB-EP001593SVG1
CSB-BP001593SVG1
CSB-MP001593SVG1
CSB-EP001593SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Acetyl-coenzyme A synthetase (acu-5), partial CSB-YP001203NHA
CSB-EP001203NHA
CSB-BP001203NHA
CSB-MP001203NHA
CSB-EP001203NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catharanthus roseus Aromatic-L-amino-acid decarboxylase (TDC) CSB-YP006583DQN
CSB-EP006583DQN
CSB-BP006583DQN
CSB-MP006583DQN
CSB-EP006583DQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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