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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Hordeum vulgare Aldose reductase CSB-YP001539HWQ
CSB-EP001539HWQ
CSB-BP001539HWQ
CSB-MP001539HWQ
CSB-EP001539HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Alpha-1D adrenergic receptor (Adra1d), partial CSB-YP001387RA1
CSB-EP001387RA1
CSB-BP001387RA1
CSB-MP001387RA1
CSB-EP001387RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Homeobox protein Hox-A7 (HOXA7) CSB-YP010657DXJ
CSB-EP010657DXJ
CSB-BP010657DXJ
CSB-MP010657DXJ
CSB-EP010657DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-galactosidase (cbgA), partial CSB-YP009476CLM
CSB-EP009476CLM
CSB-BP009476CLM
CSB-MP009476CLM
CSB-EP009476CLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echinops telfairi Hemoglobin subunit alpha (HBA) CSB-YP010147EJK
CSB-EP010147EJK
CSB-BP010147EJK
CSB-MP010147EJK
CSB-EP010147EJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain (TUB-B) CSB-YP025318EXG
CSB-EP025318EXG
CSB-BP025318EXG
CSB-MP025318EXG
CSB-EP025318EXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Ribonuclease pancreatic (RNASE1) CSB-YP019789DXU
CSB-EP019789DXU
CSB-BP019789DXU
CSB-MP019789DXU
CSB-EP019789DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta vulgaris Cytochrome c oxidase subunit 1 (COX1), partial CSB-YP015072BSQ
CSB-EP015072BSQ
CSB-BP015072BSQ
CSB-MP015072BSQ
CSB-EP015072BSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tenascin (TNC), partial CSB-YP023954HU
CSB-EP023954HU
CSB-BP023954HU
CSB-MP023954HU
CSB-EP023954HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum Cytochrome b (MT-CYB), partial CSB-YP015075DOS
CSB-EP015075DOS
CSB-BP015075DOS
CSB-MP015075DOS
CSB-EP015075DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum NADH-ubiquinone oxidoreductase chain 5 (ND5), partial CSB-YP015081DOS
CSB-EP015081DOS
CSB-BP015081DOS
CSB-MP015081DOS
CSB-EP015081DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus contorta Actin CSB-YP001205PKG
CSB-EP001205PKG
CSB-BP001205PKG
CSB-MP001205PKG
CSB-EP001205PKG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diceros bicornis Cytochrome b (MT-CYB), partial CSB-YP015075DDP
CSB-EP015075DDP
CSB-BP015075DDP
CSB-MP015075DDP
CSB-EP015075DDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072DXJ
CSB-EP015072DXJ
CSB-BP015072DXJ
CSB-MP015072DXJ
CSB-EP015072DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Balaenoptera physalus Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074BED1
CSB-EP015074BED1
CSB-BP015074BED1
CSB-MP015074BED1
CSB-EP015074BED1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisaster ochraceus NADH-ubiquinone oxidoreductase chain 5 (ND5), partial CSB-YP015081PKW
CSB-EP015081PKW
CSB-BP015081PKW
CSB-MP015081PKW
CSB-EP015081PKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Urocanate hydratase (hutU), partial CSB-YP025677FFZ
CSB-EP025677FFZ
CSB-BP025677FFZ
CSB-MP025677FFZ
CSB-EP025677FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Regulator of chromosome condensation (Bj1), partial CSB-YP019505DLU
CSB-EP019505DLU
CSB-BP019505DLU
CSB-MP019505DLU
CSB-EP019505DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S8 (RPS8A) CSB-YP020478LOG
CSB-EP020478LOG
CSB-BP020478LOG
CSB-MP020478LOG
CSB-EP020478LOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uromastyx hardwickii Alcohol dehydrogenase 1A CSB-YP001353UAV
CSB-EP001353UAV
CSB-BP001353UAV
CSB-MP001353UAV
CSB-EP001353UAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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