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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae DNA polymerase delta catalytic subunit (POL3), partial CSB-YP018303SVG
CSB-EP018303SVG
CSB-BP018303SVG
CSB-MP018303SVG
CSB-EP018303SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dromaius novaehollandiae Osteocalcin (BGLAP) CSB-YP002682DKU
CSB-EP002682DKU
CSB-BP002682DKU
CSB-MP002682DKU
CSB-EP002682DKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078FOT1
CSB-EP015078FOT1
CSB-BP015078FOT1
CSB-MP015078FOT1
CSB-EP015078FOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina NADH-ubiquinone oxidoreductase chain 2 (ND2), partial CSB-YP015077EXJ
CSB-EP015077EXJ
CSB-BP015077EXJ
CSB-MP015077EXJ
CSB-EP015077EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Uricase (Uro) CSB-YP025648DLU
CSB-EP025648DLU
CSB-BP025648DLU
CSB-MP025648DLU
CSB-EP025648DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase II subunit RPB1 (ama-1), partial CSB-YP018327CXY
CSB-EP018327CXY
CSB-BP018327CXY
CSB-MP018327CXY
CSB-EP018327CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cichlasoma nicaraguense Cytochrome b (mt-cyb), partial CSB-YP015075CKO1
CSB-EP015075CKO1
CSB-BP015075CKO1
CSB-MP015075CKO1
CSB-EP015075CKO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spalax ehrenbergi Ribonuclease pancreatic (RNASE1) CSB-YP019789FJV
CSB-EP019789FJV
CSB-BP019789FJV
CSB-MP019789FJV
CSB-EP019789FJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) CSB-YP013788HU
CSB-EP013788HU
CSB-BP013788HU
CSB-MP013788HU
CSB-EP013788HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae NADPH--cytochrome P450 reductase (NCP1), partial CSB-YP018378SVG1
CSB-EP018378SVG1
CSB-BP018378SVG1
CSB-MP018378SVG1
CSB-EP018378SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Natriuretic peptides B (NPPB) CSB-YP016021DO
CSB-EP016021DO
CSB-BP016021DO
CSB-MP016021DO
CSB-EP016021DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Thymidine kinase (TK) CSB-YP023577HSP
CSB-EP023577HSP
CSB-BP023577HSP
CSB-MP023577HSP
CSB-EP023577HSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage PRD1 Single-stranded DNA-binding protein (XII) CSB-YP022706EDO
CSB-EP022706EDO
CSB-BP022706EDO
CSB-MP022706EDO
CSB-EP022706EDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase III subunit RPC7 (RPC31) CSB-YP018349SVG
CSB-EP018349SVG
CSB-BP018349SVG
CSB-MP018349SVG
CSB-EP018349SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Proteasome subunit alpha type-4 (Pros29) CSB-YP018869DLU
CSB-EP018869DLU
CSB-BP018869DLU
CSB-MP018869DLU
CSB-EP018869DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage K11 DNA-directed RNA polymerase (1), partial CSB-YP018353ECS
CSB-EP018353ECS
CSB-BP018353ECS
CSB-MP018353ECS
CSB-EP018353ECS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Casein kinase II subunit alpha (kin-3) CSB-YP006072CXY
CSB-EP006072CXY
CSB-BP006072CXY
CSB-MP006072CXY
CSB-EP006072CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Notophthalmus viridescens Retinoic acid receptor beta (RARB) CSB-YP019339NEO
CSB-EP019339NEO
CSB-BP019339NEO
CSB-MP019339NEO
CSB-EP019339NEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum 60S acidic ribosomal protein P1 (rplp1) CSB-YP020340DKK
CSB-EP020340DKK
CSB-BP020340DKK
CSB-MP020340DKK
CSB-EP020340DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francolinus pondicerianus Hemoglobin subunit beta (HBB) CSB-YP010150FCS
CSB-EP010150FCS
CSB-BP010150FCS
CSB-MP010150FCS
CSB-EP010150FCS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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