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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Uromastyx hardwickii Hemoglobin subunit alpha-2 CSB-YP010148UAV
CSB-EP010148UAV
CSB-BP010148UAV
CSB-MP010148UAV
CSB-EP010148UAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crocuta crocuta Hemoglobin subunit beta (HBB) CSB-YP010150CXK
CSB-EP010150CXK
CSB-BP010150CXK
CSB-MP010150CXK
CSB-EP010150CXK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Hemoglobin subunit gamma-2 (HBG2) CSB-YP010156EXP
CSB-EP010156EXP
CSB-BP010156EXP
CSB-MP010156EXP
CSB-EP010156EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hippopotamus amphibius Hemoglobin subunit alpha (HBA) CSB-YP010147HHI
CSB-EP010147HHI
CSB-BP010147HHI
CSB-MP010147HHI
CSB-EP010147HHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia salina NADH-ubiquinone oxidoreductase chain 2 (ND2), partial CSB-YP015077AOE1
CSB-EP015077AOE1
CSB-BP015077AOE1
CSB-MP015077AOE1
CSB-EP015077AOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus PS3 Inorganic pyrophosphatase (ppa) CSB-YP018410BBK
CSB-EP018410BBK
CSB-BP018410BBK
CSB-MP018410BBK
CSB-EP018410BBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calsequestrin-1 (Casq1) CSB-YP004556RA
CSB-EP004556RA
CSB-BP004556RA
CSB-MP004556RA
CSB-EP004556RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paguma larvata Hemoglobin subunit beta (HBB) CSB-YP010150PAL
CSB-EP010150PAL
CSB-BP010150PAL
CSB-MP010150PAL
CSB-EP010150PAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate dehydrogenase (mdh) CSB-YP013623PLF
CSB-EP013623PLF
CSB-BP013623PLF
CSB-MP013623PLF
CSB-EP013623PLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Cytochrome c oxidase subunit 1 (COX1), partial CSB-YP015072KBK
CSB-EP015072KBK
CSB-BP015072KBK
CSB-MP015072KBK
CSB-EP015072KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxodonta africana Somatotropin (GH1) CSB-YP009407LJC
CSB-EP009407LJC
CSB-BP009407LJC
CSB-MP009407LJC
CSB-EP009407LJC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA primase large subunit (PRI2), partial CSB-YP018681SVG
CSB-EP018681SVG
CSB-BP018681SVG
CSB-MP018681SVG
CSB-EP018681SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Ribonucleoside-diphosphate reductase large subunit (I4L), partial CSB-YP020518VAA
CSB-EP020518VAA
CSB-BP020518VAA
CSB-MP020518VAA
CSB-EP020518VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus megaterium ATP synthase subunit b (atpF), partial CSB-YP002358BQW1
CSB-EP002358BQW1
CSB-BP002358BQW1
CSB-MP002358BQW1
CSB-EP002358BQW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP015080EXJ1
CSB-EP015080EXJ1
CSB-BP015080EXJ1
CSB-MP015080EXJ1
CSB-EP015080EXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus nerka NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078OBI1
CSB-EP015078OBI1
CSB-BP015078OBI1
CSB-MP015078OBI1
CSB-EP015078OBI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ras-related protein Rab-8B (rab8B) CSB-YP019223DKK
CSB-EP019223DKK
CSB-BP019223DKK
CSB-MP019223DKK
CSB-EP019223DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain CSB-YP025318ABG
CSB-EP025318ABG
CSB-BP025318ABG
CSB-MP025318ABG
CSB-EP025318ABG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ctenodactylus gundi Hemoglobin subunit beta (HBB) CSB-YP010150CTQ
CSB-EP010150CTQ
CSB-BP010150CTQ
CSB-MP010150CTQ
CSB-EP010150CTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Plasminogen activator inhibitor 1 (Serpine1) CSB-YP021081RA
CSB-BP021081RA
CSB-MP021081RA
CSB-EP021081RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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