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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vaccinia virus Protein C10 (C10L) CSB-YP003090VAA
CSB-EP003090VAA
CSB-BP003090VAA
CSB-MP003090VAA
CSB-EP003090VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Xaa-Pro dipeptidase (pepQ) CSB-YP017784ENV
CSB-EP017784ENV
CSB-BP017784ENV
CSB-MP017784ENV
CSB-EP017784ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae S-adenosylmethionine decarboxylase proenzyme (SPE2) CSB-YP001658SVG
CSB-EP001658SVG
CSB-BP001658SVG
CSB-MP001658SVG
CSB-EP001658SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uroporphyrinogen-III synthase (hemD) CSB-YP025679BRJ
CSB-EP025679BRJ
CSB-BP025679BRJ
CSB-MP025679BRJ
CSB-EP025679BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe ATP synthase protein 8 (atp8), partial CSB-YP015071SXV
CSB-EP015071SXV
CSB-BP015071SXV
CSB-MP015071SXV
CSB-EP015071SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nuclease-sensitive element-binding protein 1 (ybx1) CSB-YP026247XBE
CSB-EP026247XBE
CSB-BP026247XBE
CSB-MP026247XBE
CSB-EP026247XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varanus varius Cytochrome c CSB-YP006328VEJ
CSB-EP006328VEJ
CSB-BP006328VEJ
CSB-MP006328VEJ
CSB-EP006328VEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon jelskii Cytochrome b (MT-CYB), partial CSB-YP015075AFU
CSB-EP015075AFU
CSB-BP015075AFU
CSB-MP015075AFU
CSB-EP015075AFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon orophilus Cytochrome b (MT-CYB), partial CSB-YP015075AGA
CSB-EP015075AGA
CSB-BP015075AGA
CSB-MP015075AGA
CSB-EP015075AGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bolomys amoenus Cytochrome b (MT-CYB), partial CSB-YP015075BHY
CSB-EP015075BHY
CSB-BP015075BHY
CSB-MP015075BHY
CSB-EP015075BHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudocheirus peregrinus Lysozyme C (LYZ) CSB-YP013283PTW
CSB-EP013283PTW
CSB-BP013283PTW
CSB-MP013283PTW
CSB-EP013283PTW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Translocation protein SEC62 (SEC62), partial CSB-YP020960SVG1
CSB-EP020960SVG1
CSB-BP020960SVG1
CSB-MP020960SVG1
CSB-EP020960SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Pyruvate dehydrogenase E1 component subunit beta (pdhB) CSB-YP017717GFM
CSB-EP017717GFM
CSB-BP017717GFM
CSB-MP017717GFM
CSB-EP017717GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Pyruvate dehydrogenase E1 component subunit beta (pdhB) CSB-YP017717BRJ
CSB-EP017717BRJ
CSB-BP017717BRJ
CSB-MP017717BRJ
CSB-EP017717BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Voltage-dependent L-type calcium channel subunit alpha-1C (Cacna1c), partial CSB-YP004399RA
CSB-EP004399RA
CSB-BP004399RA
CSB-MP004399RA
CSB-EP004399RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain CSB-YP025318AVD
CSB-EP025318AVD
CSB-BP025318AVD
CSB-MP025318AVD
CSB-EP025318AVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Discopyge ommata Ras-related protein Rab-10 CSB-YP019152DES
CSB-EP019152DES
CSB-BP019152DES
CSB-MP019152DES
CSB-EP019152DES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neocallimastix frontalis Phosphoenolpyruvate carboxykinase [GTP], partial CSB-YP017614NBQ
CSB-EP017614NBQ
CSB-BP017614NBQ
CSB-MP017614NBQ
CSB-EP017614NBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Choline/ethanolaminephosphotransferase 1 (EPT1), partial CSB-YP005253SVG1
CSB-EP005253SVG1
CSB-BP005253SVG1
CSB-MP005253SVG1
CSB-EP005253SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nucleoside diphosphate kinase B (NME2), partial CSB-YP015886HU
CSB-BP015886HU
CSB-MP015886HU
CSB-EP015886HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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