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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vigna radiata var. radiata NADPH--cytochrome P450 reductase, partial CSB-YP018378VFL
CSB-EP018378VFL
CSB-BP018378VFL
CSB-MP018378VFL
CSB-EP018378VFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis LIM/homeobox protein Lhx5 (lhx5) CSB-YP012923XBE
CSB-EP012923XBE
CSB-BP012923XBE
CSB-MP012923XBE
CSB-EP012923XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP synthase subunit a (atp-6), partial CSB-YP015070NHA1
CSB-EP015070NHA1
CSB-BP015070NHA1
CSB-MP015070NHA1
CSB-EP015070NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken DNA repair protein RAD51 homolog 1 (RAD51A) CSB-YP019263CH
CSB-EP019263CH
CSB-BP019263CH
CSB-MP019263CH
CSB-EP019263CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Hypoxanthine-guanine phosphoribosyltransferase (hprT) CSB-YP010706BRJ
CSB-EP010706BRJ
CSB-BP010706BRJ
CSB-MP010706BRJ
CSB-EP010706BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium tRNA dimethylallyltransferase (miaA) CSB-YP024529SXB
CSB-EP024529SXB
CSB-BP024529SXB
CSB-MP024529SXB
CSB-EP024529SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP synthase subunit beta (atpD) CSB-YP002350BRJ
CSB-EP002350BRJ
CSB-BP002350BRJ
CSB-MP002350BRJ
CSB-EP002350BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP synthase subunit a (atpB), partial CSB-YP015070BRJ1
CSB-EP015070BRJ1
CSB-BP015070BRJ1
CSB-MP015070BRJ1
CSB-EP015070BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus G2/mitotic-specific cyclin-B1 (CCNB1) CSB-YP004806MRG
CSB-EP004806MRG
CSB-BP004806MRG
CSB-MP004806MRG
CSB-EP004806MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Aminopeptidase N (pepN), partial CSB-YP001827DQO
CSB-EP001827DQO
CSB-BP001827DQO
CSB-MP001827DQO
CSB-EP001827DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 2-oxoisovalerate dehydrogenase subunit beta (bfmBAB) CSB-YP002606BRJ
CSB-EP002606BRJ
CSB-BP002606BRJ
CSB-MP002606BRJ
CSB-EP002606BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoplasma acidophilum Inorganic pyrophosphatase (ppa) CSB-YP018410TND
CSB-EP018410TND
CSB-BP018410TND
CSB-MP018410TND
CSB-EP018410TND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA polymerase alpha subunit B (POL12), partial CSB-YP018301SVG
CSB-EP018301SVG
CSB-BP018301SVG
CSB-MP018301SVG
CSB-EP018301SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Methionine aminopeptidase 2 (MAP2) CSB-YP013716SVG
CSB-EP013716SVG
CSB-BP013716SVG
CSB-MP013716SVG
CSB-EP013716SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 14 (UBP14), partial CSB-YP025704SVG
CSB-EP025704SVG
CSB-BP025704SVG
CSB-MP025704SVG
CSB-EP025704SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Replication factor C subunit 5 (RFC5) CSB-YP019592SVG
CSB-EP019592SVG
CSB-BP019592SVG
CSB-MP019592SVG
CSB-EP019592SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UBX domain-containing protein 7 (UBX7) CSB-YP025538SVG
CSB-EP025538SVG
CSB-BP025538SVG
CSB-MP025538SVG
CSB-EP025538SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus ATP synthase protein 8 (MT-ATP8), partial CSB-YP015071HAU1
CSB-EP015071HAU1
CSB-BP015071HAU1
CSB-MP015071HAU1
CSB-EP015071HAU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072HAU
CSB-EP015072HAU
CSB-BP015072HAU
CSB-MP015072HAU
CSB-EP015072HAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077HAU
CSB-EP015077HAU
CSB-BP015077HAU
CSB-MP015077HAU
CSB-EP015077HAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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