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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 60S ribosomal protein L21 (RPL21) CSB-YP020163EKM
CSB-EP020163EKM
CSB-BP020163EKM
CSB-MP020163EKM
CSB-EP020163EKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 60S ribosomal protein L30 (RPL30) CSB-YP020225KBK
CSB-EP020225KBK
CSB-BP020225KBK
CSB-MP020225KBK
CSB-EP020225KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Exosome complex component RRP4 (RRP4) CSB-YP007890SVG
CSB-EP007890SVG
CSB-BP007890SVG
CSB-MP007890SVG
CSB-EP007890SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ADP-ribosylation factor-binding protein GGA2 (GGA2), partial CSB-YP009385SVG
CSB-EP009385SVG
CSB-BP009385SVG
CSB-MP009385SVG
CSB-EP009385SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA mismatch repair protein MLH1 (MLH1), partial CSB-YP014624SVG
CSB-EP014624SVG
CSB-BP014624SVG
CSB-MP014624SVG
CSB-EP014624SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae T-complex protein 1 subunit beta (CCT2), partial CSB-YP004856SVG
CSB-EP004856SVG
CSB-BP004856SVG
CSB-MP004856SVG
CSB-EP004856SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae T-complex protein 1 subunit zeta (CCT6), partial CSB-YP004865SVG
CSB-EP004865SVG
CSB-BP004865SVG
CSB-MP004865SVG
CSB-EP004865SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycogen phosphorylase (glgP), partial CSB-YP019123BRJ
CSB-EP019123BRJ
CSB-BP019123BRJ
CSB-MP019123BRJ
CSB-EP019123BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Isocitrate dehydrogenase [NADP] (icd) CSB-YP010990BRJ
CSB-EP010990BRJ
CSB-BP010990BRJ
CSB-MP010990BRJ
CSB-EP010990BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Xylulose kinase (xylB) CSB-YP026240BRJ
CSB-EP026240BRJ
CSB-BP026240BRJ
CSB-MP026240BRJ
CSB-EP026240BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 12 (UBP12), partial CSB-YP025699SVG
CSB-EP025699SVG
CSB-BP025699SVG
CSB-MP025699SVG
CSB-EP025699SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aldose 1-epimerase (galM) CSB-YP009200BRJ
CSB-EP009200BRJ
CSB-BP009200BRJ
CSB-MP009200BRJ
CSB-EP009200BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP010524EGI
CSB-EP010524EGI
CSB-BP010524EGI
CSB-MP010524EGI
CSB-EP010524EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Securin (PDS1) CSB-YP019074SVG
CSB-EP019074SVG
CSB-BP019074SVG
CSB-MP019074SVG
CSB-EP019074SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Low molecular weight phosphotyrosine protein phosphatase (LTP1) CSB-YP001176SVG
CSB-EP001176SVG
CSB-BP001176SVG
CSB-MP001176SVG
CSB-EP001176SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Dolichyl-phosphate beta-glucosyltransferase (ALG5), partial CSB-YP001602SVG1
CSB-EP001602SVG1
CSB-BP001602SVG1
CSB-MP001602SVG1
CSB-EP001602SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae S-formylglutathione hydrolase (YJL068C) CSB-YP007823SVG
CSB-EP007823SVG
CSB-BP007823SVG
CSB-MP007823SVG
CSB-EP007823SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe NAD-dependent malic enzyme (mae2), partial CSB-YP013633SXV
CSB-EP013633SXV
CSB-BP013633SXV
CSB-MP013633SXV
CSB-EP013633SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 7 (UBP7), partial CSB-YP025751SVG
CSB-EP025751SVG
CSB-BP025751SVG
CSB-MP025751SVG
CSB-EP025751SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein Wnt-4 (Wnt4), partial CSB-YP026137DLU
CSB-EP026137DLU
CSB-BP026137DLU
CSB-MP026137DLU
CSB-EP026137DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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