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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptomyces coelicolor Oligoribonuclease (orn) CSB-YP019586FOB
CSB-EP019586FOB
CSB-BP019586FOB
CSB-MP019586FOB
CSB-EP019586FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachemys scripta Doublesex- and mab-3-related transcription factor 1 (DMRT1) CSB-YP006969THC
CSB-EP006969THC
CSB-BP006969THC
CSB-MP006969THC
CSB-EP006969THC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoacetylglucosamine mutase (DRT101), partial CSB-YP017869DOA
CSB-EP017869DOA
CSB-BP017869DOA
CSB-MP017869DOA
CSB-EP017869DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Estrogen receptor beta (esr2), partial CSB-YP007831OEI
CSB-EP007831OEI
CSB-BP007831OEI
CSB-MP007831OEI
CSB-EP007831OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sestrin-1 (sesn1) CSB-YP021095XBE
CSB-EP021095XBE
CSB-BP021095XBE
CSB-MP021095XBE
CSB-EP021095XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis mossambicus Plasma membrane calcium-transporting ATPase 2 (atp2b2), partial CSB-YP002336OES
CSB-EP002336OES
CSB-BP002336OES
CSB-MP002336OES
CSB-EP002336OES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoplasma volcanium Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP017614TDL
CSB-EP017614TDL
CSB-BP017614TDL
CSB-MP017614TDL
CSB-EP017614TDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UTP--glucose-1-phosphate uridylyltransferase (cap4C) CSB-YP025569FMW
CSB-EP025569FMW
CSB-BP025569FMW
CSB-MP025569FMW
CSB-EP025569FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-mercaptopyruvate sulfurtransferase (sseA) CSB-YP014770EOD
CSB-EP014770EOD
CSB-BP014770EOD
CSB-MP014770EOD
CSB-EP014770EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Frizzled-5 (fzd5), partial CSB-YP009108XBE1
CSB-EP009108XBE1
CSB-BP009108XBE1
CSB-MP009108XBE1
CSB-EP009108XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori tRNA pseudouridine synthase A (truA) CSB-YP019086HUV
CSB-EP019086HUV
CSB-BP019086HUV
CSB-MP019086HUV
CSB-EP019086HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribulose-phosphate 3-epimerase (rpe) CSB-YP020097HUV
CSB-EP020097HUV
CSB-BP020097HUV
CSB-MP020097HUV
CSB-EP020097HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops C-C chemokine receptor type 3 (CCR3), partial CSB-YP004842DSU1
CSB-EP004842DSU1
CSB-BP004842DSU1
CSB-MP004842DSU1
CSB-EP004842DSU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b (cob), partial CSB-YP015075AVD1
CSB-EP015075AVD1
CSB-BP015075AVD1
CSB-MP015075AVD1
CSB-EP015075AVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox L-amino-acid oxidase CSB-YP011660DYC
CSB-EP011660DYC
CSB-BP011660DYC
CSB-MP011660DYC
CSB-EP011660DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Eukaryotic translation initiation factor 3 subunit D (TIF3D1), partial CSB-YP007533DOA
CSB-EP007533DOA
CSB-BP007533DOA
CSB-MP007533DOA
CSB-EP007533DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824RLE
CSB-EP005824RLE
CSB-BP005824RLE
CSB-MP005824RLE
CSB-EP005824RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Argininosuccinate synthase (argG) CSB-YP002234BWZ
CSB-EP002234BWZ
CSB-BP002234BWZ
CSB-MP002234BWZ
CSB-EP002234BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Superoxide dismutase [Mn] (sodA) CSB-YP022398BWZ
CSB-EP022398BWZ
CSB-BP022398BWZ
CSB-MP022398BWZ
CSB-EP022398BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Thymidylate kinase (tmk) CSB-YP007226BWZ
CSB-EP007226BWZ
CSB-BP007226BWZ
CSB-MP007226BWZ
CSB-EP007226BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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