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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Hordeum vulgare Profilin-1 (PRO1) CSB-YP017824HWQ
CSB-EP017824HWQ
CSB-BP017824HWQ
CSB-MP017824HWQ
CSB-EP017824HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiosulfate sulfurtransferase (rhdA) CSB-YP025197DPX
CSB-EP025197DPX
CSB-BP025197DPX
CSB-MP025197DPX
CSB-EP025197DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Importin subunit alpha-5 (KPNA1) CSB-YP012483HU
CSB-EP012483HU
CSB-BP012483HU
CSB-MP012483HU
CSB-EP012483HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nuclear cap-binding protein subunit 2 (ncbp2) CSB-YP015521XBE
CSB-EP015521XBE
CSB-BP015521XBE
CSB-MP015521XBE
CSB-EP015521XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Proteasome subunit alpha type-4 (PAC1) CSB-YP018869FKI
CSB-EP018869FKI
CSB-BP018869FKI
CSB-MP018869FKI
CSB-EP018869FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L23 (RPL23) CSB-YP020186OEG
CSB-EP020186OEG
CSB-BP020186OEG
CSB-MP020186OEG
CSB-EP020186OEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L22 (rpl-22) CSB-YP020182CXY
CSB-EP020182CXY
CSB-BP020182CXY
CSB-MP020182CXY
CSB-EP020182CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema hyodysenteriae Elongation factor Tu (tuf) CSB-YP025342TPQ
CSB-EP025342TPQ
CSB-BP025342TPQ
CSB-MP025342TPQ
CSB-EP025342TPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Alanine aminotransferase 2 CSB-YP009865HWQ
CSB-EP009865HWQ
CSB-BP009865HWQ
CSB-MP009865HWQ
CSB-EP009865HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717SSQ
CSB-EP009717SSQ
CSB-BP009717SSQ
CSB-MP009717SSQ
CSB-EP009717SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharopolyspora erythraea Propionyl-CoA carboxylase beta chain (pccB), partial CSB-YP017523STB
CSB-EP017523STB
CSB-BP017523STB
CSB-MP017523STB
CSB-EP017523STB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM13 (TIM13) CSB-YP023549SVG
CSB-EP023549SVG
CSB-BP023549SVG
CSB-MP023549SVG
CSB-EP023549SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhynchosporium secalis Tubulin beta chain CSB-YP025318REH
CSB-EP025318REH
CSB-BP025318REH
CSB-MP025318REH
CSB-EP025318REH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Proto-oncogene c-Fos (fos) CSB-YP008790TKY
CSB-EP008790TKY
CSB-BP008790TKY
CSB-MP008790TKY
CSB-EP008790TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Actin (HCBG_08711) CSB-YP001205AYZ
CSB-EP001205AYZ
CSB-BP001205AYZ
CSB-MP001205AYZ
CSB-EP001205AYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba castellanii Actin-related protein 3 (ARP3) CSB-YP001249AAJ
CSB-EP001249AAJ
CSB-BP001249AAJ
CSB-MP001249AAJ
CSB-EP001249AAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clarias gariepinus Glycoprotein hormones alpha chain (cga) CSB-YP005293DTT
CSB-EP005293DTT
CSB-BP005293DTT
CSB-MP005293DTT
CSB-EP005293DTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917EZX
CSB-EP022917EZX
CSB-BP022917EZX
CSB-MP022917EZX
CSB-EP022917EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MKY
CSB-EP022398MKY
CSB-BP022398MKY
CSB-MP022398MKY
CSB-EP022398MKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MVW
CSB-EP022398MVW
CSB-BP022398MVW
CSB-MP022398MVW
CSB-EP022398MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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