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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Actin-related protein 3 (ARP3) CSB-YP001249SVG
CSB-EP001249SVG
CSB-BP001249SVG
CSB-MP001249SVG
CSB-EP001249SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-complex protein 1 subunit epsilon (cct-5), partial CSB-YP004862CXY
CSB-EP004862CXY
CSB-BP004862CXY
CSB-MP004862CXY
CSB-EP004862CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Paired box protein Pax-6 (PAX6) CSB-YP017492DXJ
CSB-EP017492DXJ
CSB-BP017492DXJ
CSB-MP017492DXJ
CSB-EP017492DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Elongation factor Ts (tsf) CSB-YP025124MLN
CSB-EP025124MLN
CSB-BP025124MLN
CSB-MP025124MLN
CSB-EP025124MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Thymidine kinase (tdk) CSB-YP023577MLN
CSB-EP023577MLN
CSB-BP023577MLN
CSB-MP023577MLN
CSB-EP023577MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium ATP synthase subunit a (atpB), partial CSB-YP015070MLN1
CSB-EP015070MLN1
CSB-BP015070MLN1
CSB-MP015070MLN1
CSB-EP015070MLN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Non-secretory ribonuclease (RNASE2) CSB-YP019794GGZ
CSB-EP019794GGZ
CSB-BP019794GGZ
CSB-MP019794GGZ
CSB-EP019794GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taeniopygia guttata Myelin proteolipid protein (PLP1), partial CSB-YP018202TKW1
CSB-EP018202TKW1
CSB-BP018202TKW1
CSB-MP018202TKW1
CSB-EP018202TKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Peptide chain release factor 1 (prfA) CSB-YP015197FMN
CSB-EP015197FMN
CSB-BP015197FMN
CSB-MP015197FMN
CSB-EP015197FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyberlindnera mrakii Cytochrome c oxidase subunit 2 (COX2), partial CSB-YP015073CVB1
CSB-EP015073CVB1
CSB-BP015073CVB1
CSB-MP015073CVB1
CSB-EP015073CVB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S3 (rps-3) CSB-YP020443CXY
CSB-EP020443CXY
CSB-BP020443CXY
CSB-MP020443CXY
CSB-EP020443CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Cytochrome c oxidase subunit 1 (mt-co1), partial CSB-YP015072OEI
CSB-EP015072OEI
CSB-BP015072OEI
CSB-MP015072OEI
CSB-EP015072OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss NADH-ubiquinone oxidoreductase chain 6 (MT-ND6), partial CSB-YP015082OEI1
CSB-EP015082OEI1
CSB-BP015082OEI1
CSB-MP015082OEI1
CSB-EP015082OEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Serine palmitoyltransferase 2 (LCB2), partial CSB-YP022640KBK1
CSB-EP022640KBK1
CSB-BP022640KBK1
CSB-MP022640KBK1
CSB-EP022640KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant V-type proton ATPase subunit B CSB-YP002398DZV
CSB-EP002398DZV
CSB-BP002398DZV
CSB-MP002398DZV
CSB-EP002398DZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Cytochrome b-c1 complex subunit 7 CSB-YP025664FIG
CSB-EP025664FIG
CSB-BP025664FIG
CSB-MP025664FIG
CSB-EP025664FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon siberiae Cytochrome b (MT-CYB), partial CSB-YP015075AGC
CSB-EP015075AGC
CSB-BP015075AGC
CSB-MP015075AGC
CSB-EP015075AGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces marxianus 60S ribosomal protein L39 (RPL39) CSB-YP020277KAN
CSB-EP020277KAN
CSB-BP020277KAN
CSB-MP020277KAN
CSB-EP020277KAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 40S ribosomal protein S25 (RpS25) CSB-YP020406DLU
CSB-EP020406DLU
CSB-BP020406DLU
CSB-MP020406DLU
CSB-EP020406DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia prowazekii Elongation factor Tu (tuf) CSB-YP025342RMY
CSB-EP025342RMY
CSB-BP025342RMY
CSB-MP025342RMY
CSB-EP025342RMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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