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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cytochrome c oxidase subunit 3 (COX3), partial CSB-YP015074DZV1
CSB-EP015074DZV1
CSB-BP015074DZV1
CSB-MP015074DZV1
CSB-EP015074DZV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Albinaria coerulea Cytochrome c oxidase subunit 2 (COII), partial CSB-YP015073AGI1
CSB-EP015073AGI1
CSB-BP015073AGI1
CSB-MP015073AGI1
CSB-EP015073AGI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Albinaria coerulea NADH-ubiquinone oxidoreductase chain 1 (ND1), partial CSB-YP015076AGI
CSB-EP015076AGI
CSB-BP015076AGI
CSB-MP015076AGI
CSB-EP015076AGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia canadensis NADH-ubiquinone oxidoreductase chain 2 (ND2), partial CSB-YP015077PJU
CSB-EP015077PJU
CSB-BP015077PJU
CSB-MP015077PJU
CSB-EP015077PJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia canadensis NADH-ubiquinone oxidoreductase chain 4 (ND4), partial CSB-YP015079PJU
CSB-EP015079PJU
CSB-BP015079PJU
CSB-MP015079PJU
CSB-EP015079PJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chondrus crispus NADH-ubiquinone oxidoreductase chain 6 (ND6), partial CSB-YP015082CJR1
CSB-EP015082CJR1
CSB-BP015082CJR1
CSB-MP015082CJR1
CSB-EP015082CJR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinate dehydrogenase [ubiquinone] iron-sulfur subunit (SDH2) CSB-YP020907DZV
CSB-EP020907DZV
CSB-BP020907DZV
CSB-MP020907DZV
CSB-EP020907DZV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L35a (rpl-33) CSB-YP020248CXY
CSB-EP020248CXY
CSB-BP020248CXY
CSB-MP020248CXY
CSB-EP020248CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 40S ribosomal protein S13 (rps13) CSB-YP020370XBE
CSB-EP020370XBE
CSB-BP020370XBE
CSB-MP020370XBE
CSB-EP020370XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba castellanii Ubiquitin-60S ribosomal protein L40 CSB-YP025416AAJ
CSB-EP025416AAJ
CSB-BP025416AAJ
CSB-MP025416AAJ
CSB-EP025416AAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus G protein-activated inward rectifier potassium channel 2 (KCNJ6), partial CSB-YP012059MRG1
CSB-EP012059MRG1
CSB-BP012059MRG1
CSB-MP012059MRG1
CSB-EP012059MRG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyura stolonifera 60S ribosomal protein L21 (RPL21) CSB-YP020163PXZ
CSB-EP020163PXZ
CSB-BP020163PXZ
CSB-MP020163PXZ
CSB-EP020163PXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Glucocorticoid receptor (nr3c1), partial CSB-YP016059OEI
CSB-EP016059OEI
CSB-BP016059OEI
CSB-MP016059OEI
CSB-EP016059OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transcription initiation factor TFIID subunit 12 (Taf12) CSB-YP023079DLU
CSB-EP023079DLU
CSB-BP023079DLU
CSB-MP023079DLU
CSB-EP023079DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP002355FOI
CSB-EP002355FOI
CSB-BP002355FOI
CSB-MP002355FOI
CSB-EP002355FOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Elongation factor Tu (tufA) CSB-YP025342GCI
CSB-EP025342GCI
CSB-BP025342GCI
CSB-MP025342GCI
CSB-EP025342GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 8 (UBP8) CSB-YP025752SVG
CSB-EP025752SVG
CSB-BP025752SVG
CSB-MP025752SVG
CSB-EP025752SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis T-complex protein 1 subunit gamma (cct3), partial CSB-YP004857XBE
CSB-EP004857XBE
CSB-BP004857XBE
CSB-MP004857XBE
CSB-EP004857XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ambystoma mexicanum Homeobox protein Hox-A13 (HOXA13) CSB-YP010651BYD
CSB-EP010651BYD
CSB-BP010651BYD
CSB-MP010651BYD
CSB-EP010651BYD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Putative adenosylhomocysteinase 2 (Ahcy89E) CSB-YP001475DLU
CSB-EP001475DLU
CSB-BP001475DLU
CSB-MP001475DLU
CSB-EP001475DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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