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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Translation initiation factor IF-2 (infB), partial CSB-YP015164BWZ
CSB-EP015164BWZ
CSB-BP015164BWZ
CSB-MP015164BWZ
CSB-EP015164BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150BWZ
CSB-EP015150BWZ
CSB-BP015150BWZ
CSB-MP015150BWZ
CSB-EP015150BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Single-stranded DNA-binding protein (ssb) CSB-YP022706BWZ
CSB-EP022706BWZ
CSB-BP022706BWZ
CSB-MP022706BWZ
CSB-EP022706BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Oligoribonuclease (orn) CSB-YP019586EZX
CSB-EP019586EZX
CSB-BP019586EZX
CSB-MP019586EZX
CSB-EP019586EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U6 snRNA-associated Sm-like protein LSm3 (LSM3) CSB-YP013206SVG
CSB-EP013206SVG
CSB-BP013206SVG
CSB-MP013206SVG
CSB-EP013206SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Acyl-CoA-binding domain-containing protein 6 (ACBP6) CSB-YP001139DOA
CSB-EP001139DOA
CSB-BP001139DOA
CSB-MP001139DOA
CSB-EP001139DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Cysteine desulfurase (iscS) CSB-YP015769ESG
CSB-EP015769ESG
CSB-BP015769ESG
CSB-MP015769ESG
CSB-EP015769ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Guanylate kinase (gmk) CSB-YP010059ESG
CSB-EP010059ESG
CSB-BP010059ESG
CSB-MP010059ESG
CSB-EP010059ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Lipoyl synthase (lipA) CSB-YP012927ESG
CSB-EP012927ESG
CSB-BP012927ESG
CSB-MP012927ESG
CSB-EP012927ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Translation initiation factor IF-2 (infB), partial CSB-YP015164LNG
CSB-EP015164LNG
CSB-BP015164LNG
CSB-MP015164LNG
CSB-EP015164LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus TATA-box-binding protein (tbp) CSB-YP023239FPM
CSB-EP023239FPM
CSB-BP023239FPM
CSB-MP023239FPM
CSB-EP023239FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Pterin-4-alpha-carbinolamine dehydratase (Pcd) CSB-YP017514DMP
CSB-EP017514DMP
CSB-BP017514DMP
CSB-MP017514DMP
CSB-EP017514DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Copper-transporting ATPase 1 (actP1), partial CSB-YP002414RKU
CSB-EP002414RKU
CSB-BP002414RKU
CSB-MP002414RKU
CSB-EP002414RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurus punctatus 60S ribosomal protein L30 (rpl30) CSB-YP020225ILX
CSB-EP020225ILX
CSB-BP020225ILX
CSB-MP020225ILX
CSB-EP020225ILX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Single-stranded DNA-binding protein (ssb) CSB-YP022706HSY
CSB-EP022706HSY
CSB-BP022706HSY
CSB-MP022706HSY
CSB-EP022706HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Actin (ACT1) CSB-YP001205SVG
CSB-BP001205SVG
CSB-MP001205SVG
CSB-EP001205SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes 5-hydroxytryptamine receptor 1B (HTR1B), partial CSB-YP010882EQV1
CSB-EP010882EQV1
CSB-BP010882EQV1
CSB-MP010882EQV1
CSB-EP010882EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Neutrophil defensin 1 CSB-YP006652MOW
CSB-EP006652MOW
CSB-BP006652MOW
CSB-MP006652MOW
CSB-EP006652MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylobates lar Polyadenylate-binding protein 5 (PABPC5) CSB-YP017359HXW
CSB-EP017359HXW
CSB-BP017359HXW
CSB-MP017359HXW
CSB-EP017359HXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP019086SJV
CSB-EP019086SJV
CSB-BP019086SJV
CSB-MP019086SJV
CSB-EP019086SJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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