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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Notophthalmus viridescens Homeobox protein DLX-3 (DLX3) CSB-YP006954NEO
CSB-EP006954NEO
CSB-BP006954NEO
CSB-MP006954NEO
CSB-EP006954NEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis G1/S-specific cyclin-D2 (ccnd2) CSB-YP004812XBE
CSB-EP004812XBE
CSB-BP004812XBE
CSB-MP004812XBE
CSB-EP004812XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Squalene synthase (SQS1), partial CSB-YP008562DOA1
CSB-EP008562DOA1
CSB-BP008562DOA1
CSB-MP008562DOA1
CSB-EP008562DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U3 small nucleolar ribonucleoprotein protein IMP4 (IMP4) CSB-YP011693SVG
CSB-EP011693SVG
CSB-BP011693SVG
CSB-MP011693SVG
CSB-EP011693SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Isocitrate dehydrogenase [NADP] (IDP3) CSB-YP010990SVG
CSB-EP010990SVG
CSB-BP010990SVG
CSB-MP010990SVG
CSB-EP010990SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ampullariella sp. Xylulose kinase (xylB) CSB-YP026240AJC
CSB-EP026240AJC
CSB-BP026240AJC
CSB-MP026240AJC
CSB-EP026240AJC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Voltage-dependent L-type calcium channel subunit beta-3 (Cacnb3) CSB-YP004413RA
CSB-EP004413RA
CSB-BP004413RA
CSB-MP004413RA
CSB-EP004413RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin gamma-2 chain CSB-YP025336EPN
CSB-EP025336EPN
CSB-BP025336EPN
CSB-MP025336EPN
CSB-EP025336EPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum AP-2 complex subunit mu (apm2) CSB-YP001872DKK
CSB-EP001872DKK
CSB-BP001872DKK
CSB-MP001872DKK
CSB-EP001872DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ephrin type-A receptor 5 (EPHA5), partial CSB-YP007725HU
CSB-EP007725HU
CSB-BP007725HU
CSB-MP007725HU
CSB-EP007725HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-CoA acetyltransferase (phaA) CSB-YP001134PBZ
CSB-EP001134PBZ
CSB-BP001134PBZ
CSB-MP001134PBZ
CSB-EP001134PBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydroxymethylglutaryl-CoA synthase (F25B4.6) CSB-YP010567CXY
CSB-EP010567CXY
CSB-BP010567CXY
CSB-MP010567CXY
CSB-EP010567CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Inositol monophosphatase 1 (IMP1) CSB-YP011694SZY
CSB-EP011694SZY
CSB-BP011694SZY
CSB-MP011694SZY
CSB-EP011694SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UDP-glucose 4-epimerase (galE) CSB-YP009197BRJ
CSB-EP009197BRJ
CSB-BP009197BRJ
CSB-MP009197BRJ
CSB-EP009197BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Caspase-3 (Casp3) CSB-YP004548RA
CSB-EP004548RA
CSB-BP004548RA
CSB-MP004548RA
CSB-EP004548RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crocodylus novaeguineae Somatotropin (GH) CSB-YP009407CSK
CSB-EP009407CSK
CSB-BP009407CSK
CSB-MP009407CSK
CSB-EP009407CSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076GER
CSB-EP015076GER
CSB-BP015076GER
CSB-MP015076GER
CSB-EP015076GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Translation initiation factor IF-3 (infC) CSB-YP015166HUV
CSB-EP015166HUV
CSB-BP015166HUV
CSB-MP015166HUV
CSB-EP015166HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP synthase subunit alpha (atpA), partial CSB-YP002344HUV
CSB-EP002344HUV
CSB-BP002344HUV
CSB-MP002344HUV
CSB-EP002344HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Elongation factor Tu (tuf) CSB-YP025342HUV
CSB-EP025342HUV
CSB-BP025342HUV
CSB-MP025342HUV
CSB-EP025342HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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