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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Suberites domuncula V-type proton ATPase subunit D (VATPD) CSB-YP002401FOW
CSB-EP002401FOW
CSB-BP002401FOW
CSB-MP002401FOW
CSB-EP002401FOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Ferrochelatase (hemH) CSB-YP008579ZBM
CSB-EP008579ZBM
CSB-BP008579ZBM
CSB-MP008579ZBM
CSB-EP008579ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Translation initiation factor IF-2 (infB), partial CSB-YP015164ESG
CSB-EP015164ESG
CSB-BP015164ESG
CSB-MP015164ESG
CSB-EP015164ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Phosphoserine aminotransferase (serC) CSB-YP018838ESG
CSB-EP018838ESG
CSB-BP018838ESG
CSB-MP018838ESG
CSB-EP018838ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Thymidine kinase (tdk) CSB-YP023577ESG
CSB-EP023577ESG
CSB-BP023577ESG
CSB-MP023577ESG
CSB-EP023577ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Methionyl-tRNA formyltransferase (fmt) CSB-YP015150ESG
CSB-EP015150ESG
CSB-BP015150ESG
CSB-MP015150ESG
CSB-EP015150ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Ribosome-recycling factor (frr) CSB-YP014941ESG
CSB-EP014941ESG
CSB-BP014941ESG
CSB-MP014941ESG
CSB-EP014941ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus GTPase Era (era) CSB-YP007759DQO
CSB-EP007759DQO
CSB-BP007759DQO
CSB-MP007759DQO
CSB-EP007759DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alces alces alces Melanocyte-stimulating hormone receptor (MC1R), partial CSB-YP013558AZJ1
CSB-EP013558AZJ1
CSB-BP013558AZJ1
CSB-MP013558AZJ1
CSB-EP013558AZJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (Ogt), partial CSB-YP016315RA
CSB-EP016315RA
CSB-BP016315RA
CSB-MP016315RA
CSB-EP016315RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Myc-associated zinc finger protein (MAZ) CSB-YP013528MRG
CSB-EP013528MRG
CSB-BP013528MRG
CSB-MP013528MRG
CSB-EP013528MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasyatis akajei Hemoglobin subunit beta (HBB) CSB-YP010150DAL
CSB-EP010150DAL
CSB-BP010150DAL
CSB-MP010150DAL
CSB-EP010150DAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Phosphoserine aminotransferase (serC) CSB-YP018838NGG
CSB-EP018838NGG
CSB-BP018838NGG
CSB-MP018838NGG
CSB-EP018838NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Maleylacetoacetate isomerase (maiA) CSB-YP009994EZX
CSB-EP009994EZX
CSB-BP009994EZX
CSB-MP009994EZX
CSB-EP009994EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Peptide chain release factor 1 (prfA) CSB-YP015197BWZ
CSB-EP015197BWZ
CSB-BP015197BWZ
CSB-MP015197BWZ
CSB-EP015197BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (aceF) CSB-YP006926BWZ
CSB-EP006926BWZ
CSB-BP006926BWZ
CSB-MP006926BWZ
CSB-EP006926BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Thymidylate synthase (thyA) CSB-YP025393BWZ
CSB-EP025393BWZ
CSB-BP025393BWZ
CSB-MP025393BWZ
CSB-EP025393BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824BWZ
CSB-EP005824BWZ
CSB-BP005824BWZ
CSB-MP005824BWZ
CSB-EP005824BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Peptide deformylase (def) CSB-YP017707BWZ
CSB-EP017707BWZ
CSB-BP017707BWZ
CSB-MP017707BWZ
CSB-EP017707BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum tRNA dimethylallyltransferase (miaA) CSB-YP024529BWZ
CSB-EP024529BWZ
CSB-BP024529BWZ
CSB-MP024529BWZ
CSB-EP024529BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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