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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Trichechus inunguis Hemoglobin subunit alpha (HBA) CSB-YP010147THX
CSB-EP010147THX
CSB-BP010147THX
CSB-MP010147THX
CSB-EP010147THX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasyurus viverrinus Hemoglobin subunit beta (HBB) CSB-YP010150DAR
CSB-EP010150DAR
CSB-BP010150DAR
CSB-MP010150DAR
CSB-EP010150DAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lama vicugna Hemoglobin subunit alpha (HBA) CSB-YP010147LBV
CSB-EP010147LBV
CSB-BP010147LBV
CSB-MP010147LBV
CSB-EP010147LBV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus borealis Hemoglobin subunit alpha-2 (hba2) CSB-YP010148XAO
CSB-EP010148XAO
CSB-BP010148XAO
CSB-MP010148XAO
CSB-EP010148XAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physarum polycephalum Tubulin beta-1 chain (BETA) CSB-YP025319EVC
CSB-EP025319EVC
CSB-BP025319EVC
CSB-MP025319EVC
CSB-EP025319EVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus rufogriseus Alpha-lactalbumin (LALBA) CSB-YP012724MAL
CSB-EP012724MAL
CSB-BP012724MAL
CSB-MP012724MAL
CSB-EP012724MAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Superoxide dismutase [Cu-Zn] (sod-1) CSB-YP022397NHA
CSB-EP022397NHA
CSB-BP022397NHA
CSB-MP022397NHA
CSB-EP022397NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi3T Thymidylate synthase (thyP3) CSB-YP025393BBD
CSB-EP025393BBD
CSB-BP025393BBD
CSB-MP025393BBD
CSB-EP025393BBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Serum amyloid P-component (APCS) CSB-YP001898MRG
CSB-EP001898MRG
CSB-BP001898MRG
CSB-MP001898MRG
CSB-EP001898MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Superoxide dismutase [Cu-Zn] (Sod1) CSB-BP022397RA
CSB-MP022397RA
CSB-EP022397RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cytochrome c oxidase subunit 1 (cox1), partial CSB-YP015072SXV
CSB-EP015072SXV
CSB-BP015072SXV
CSB-MP015072SXV
CSB-EP015072SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T3 DNA-directed RNA polymerase (1), partial CSB-YP018353EDY
CSB-EP018353EDY
CSB-BP018353EDY
CSB-MP018353EDY
CSB-EP018353EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus PS3 ATP synthase subunit beta (atpD) CSB-YP002350BBK
CSB-EP002350BBK
CSB-BP002350BBK
CSB-MP002350BBK
CSB-EP002350BBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Epoxide hydrolase 1 (Ephx1), partial CSB-YP007734RA1
CSB-EP007734RA1
CSB-BP007734RA1
CSB-MP007734RA1
CSB-EP007734RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torpedo marmorata Acetylcholinesterase (ache), partial CSB-YP001154TGV
CSB-EP001154TGV
CSB-BP001154TGV
CSB-MP001154TGV
CSB-EP001154TGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerases I and III subunit RPAC1 (RPC40) CSB-YP018324SVG
CSB-EP018324SVG
CSB-BP018324SVG
CSB-MP018324SVG
CSB-EP018324SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba NADH-ubiquinone oxidoreductase chain 3 (mt:ND3), partial CSB-YP015078DMR1
CSB-EP015078DMR1
CSB-BP015078DMR1
CSB-MP015078DMR1
CSB-EP015078DMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba NADH-ubiquinone oxidoreductase chain 4 (mt:ND4), partial CSB-YP015079DMR
CSB-EP015079DMR
CSB-BP015079DMR
CSB-MP015079DMR
CSB-EP015079DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba NADH-ubiquinone oxidoreductase chain 6 (mt:ND6), partial CSB-YP015082DMR1
CSB-EP015082DMR1
CSB-BP015082DMR1
CSB-MP015082DMR1
CSB-EP015082DMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiobacillus ferrooxidans Glutamine synthetase (glnA) CSB-YP009553TOA
CSB-EP009553TOA
CSB-BP009553TOA
CSB-MP009553TOA
CSB-EP009553TOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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