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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma mobile Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP740295MAAC
CSB-EP740295MAAC
CSB-BP740295MAAC
CSB-MP740295MAAC
CSB-EP740295MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Elongation factor G (fusA), partial CSB-YP740296MAAC
CSB-EP740296MAAC
CSB-BP740296MAAC
CSB-MP740296MAAC
CSB-EP740296MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 50S ribosomal protein L21 (rplU) CSB-YP740297MAAC
CSB-EP740297MAAC
CSB-BP740297MAAC
CSB-MP740297MAAC
CSB-EP740297MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Translation initiation factor IF-1 (infA) CSB-YP740298MAAC
CSB-EP740298MAAC
CSB-BP740298MAAC
CSB-MP740298MAAC
CSB-EP740298MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 30S ribosomal protein S14 type Z (rpsZ) CSB-YP740299MAAC
CSB-EP740299MAAC
CSB-BP740299MAAC
CSB-MP740299MAAC
CSB-EP740299MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 50S ribosomal protein L29 (rpmC) CSB-YP740300MAAC
CSB-EP740300MAAC
CSB-BP740300MAAC
CSB-MP740300MAAC
CSB-EP740300MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 30S ribosomal protein S18 (rpsR) CSB-YP740301MAAC
CSB-EP740301MAAC
CSB-BP740301MAAC
CSB-MP740301MAAC
CSB-EP740301MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile ATP synthase subunit alpha (atpA), partial CSB-YP740302MAAC
CSB-EP740302MAAC
CSB-BP740302MAAC
CSB-MP740302MAAC
CSB-EP740302MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Peptide chain release factor 1 (prfA) CSB-YP740303MAAC
CSB-EP740303MAAC
CSB-BP740303MAAC
CSB-MP740303MAAC
CSB-EP740303MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP740304MAAC
CSB-EP740304MAAC
CSB-BP740304MAAC
CSB-MP740304MAAC
CSB-EP740304MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Ribosome maturation factor RimP (rimP) CSB-YP740305MAAC
CSB-EP740305MAAC
CSB-BP740305MAAC
CSB-MP740305MAAC
CSB-EP740305MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile 50S ribosomal protein L11 (rplK) CSB-YP740306MAAC
CSB-EP740306MAAC
CSB-BP740306MAAC
CSB-MP740306MAAC
CSB-EP740306MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Protein translocase subunit SecA (secA), partial CSB-YP740307MAAC
CSB-EP740307MAAC
CSB-BP740307MAAC
CSB-MP740307MAAC
CSB-EP740307MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile UPF0133 protein MMOB0740 (MMOB0740) CSB-YP740308MAAC
CSB-EP740308MAAC
CSB-BP740308MAAC
CSB-MP740308MAAC
CSB-EP740308MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma mobile Lysine--tRNA ligase (lysS) CSB-YP740309MAAC
CSB-EP740309MAAC
CSB-BP740309MAAC
CSB-MP740309MAAC
CSB-EP740309MAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP740310BQE
CSB-EP740310BQE
CSB-BP740310BQE
CSB-MP740310BQE
CSB-EP740310BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Peptide chain release factor subunit 1 (prf1) CSB-YP740311PAAB
CSB-EP740311PAAB
CSB-BP740311PAAB
CSB-MP740311PAAB
CSB-EP740311PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Proline--tRNA ligase (proS) CSB-YP740312PAAB
CSB-EP740312PAAB
CSB-BP740312PAAB
CSB-MP740312PAAB
CSB-EP740312PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus Nucleoside diphosphate kinase (ndk) CSB-YP740313PAAB
CSB-EP740313PAAB
CSB-BP740313PAAB
CSB-MP740313PAAB
CSB-EP740313PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picrophilus torridus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP740314PAAB
CSB-EP740314PAAB
CSB-BP740314PAAB
CSB-MP740314PAAB
CSB-EP740314PAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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