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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Diaminopimelate epimerase (dapF) CSB-YP742737EYO
CSB-EP742737EYO
CSB-BP742737EYO
CSB-MP742737EYO
CSB-EP742737EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein thf1 (thf1) CSB-YP742738EYO
CSB-EP742738EYO
CSB-BP742738EYO
CSB-MP742738EYO
CSB-EP742738EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP742739EYO
CSB-EP742739EYO
CSB-BP742739EYO
CSB-MP742739EYO
CSB-EP742739EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP742740EYO
CSB-EP742740EYO
CSB-BP742740EYO
CSB-MP742740EYO
CSB-EP742740EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP742741EYO
CSB-EP742741EYO
CSB-BP742741EYO
CSB-MP742741EYO
CSB-EP742741EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP742742EYO
CSB-EP742742EYO
CSB-BP742742EYO
CSB-MP742742EYO
CSB-EP742742EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Imidazole glycerol phosphate synthase subunit hisF1 (hisF1) CSB-YP742743EYO
CSB-EP742743EYO
CSB-BP742743EYO
CSB-MP742743EYO
CSB-EP742743EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA repair protein recO (recO) CSB-YP742744EYO
CSB-EP742744EYO
CSB-BP742744EYO
CSB-MP742744EYO
CSB-EP742744EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Undecaprenyl-diphosphatase (uppP), partial CSB-YP742745EYO
CSB-EP742745EYO
CSB-BP742745EYO
CSB-MP742745EYO
CSB-EP742745EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA (Ile)-lysidine synthase (tilS) CSB-YP742746EYO
CSB-EP742746EYO
CSB-BP742746EYO
CSB-MP742746EYO
CSB-EP742746EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP742747EYO
CSB-EP742747EYO
CSB-BP742747EYO
CSB-MP742747EYO
CSB-EP742747EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chaperone protein DnaJ (dnaJ) CSB-YP742748EYO
CSB-EP742748EYO
CSB-BP742748EYO
CSB-MP742748EYO
CSB-EP742748EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glycine cleavage system H protein (gcvH) CSB-YP742749EYP
CSB-EP742749EYP
CSB-BP742749EYP
CSB-MP742749EYP
CSB-EP742749EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA (Ile)-lysidine synthase (tilS) CSB-YP742750EYP
CSB-EP742750EYP
CSB-BP742750EYP
CSB-MP742750EYP
CSB-EP742750EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adenosylhomocysteinase (ahcY) CSB-YP742751EYP
CSB-EP742751EYP
CSB-BP742751EYP
CSB-MP742751EYP
CSB-EP742751EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional pantoate ligase/cytidylate kinase (panC/cmk), partial CSB-YP742752EYP
CSB-EP742752EYP
CSB-BP742752EYP
CSB-MP742752EYP
CSB-EP742752EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S3 (rpsC) CSB-YP742753EYP
CSB-EP742753EYP
CSB-BP742753EYP
CSB-MP742753EYP
CSB-EP742753EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S8 (rpsH) CSB-YP742754EYP
CSB-EP742754EYP
CSB-BP742754EYP
CSB-MP742754EYP
CSB-EP742754EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S11 (rpsK) CSB-YP742755EYP
CSB-EP742755EYP
CSB-BP742755EYP
CSB-MP742755EYP
CSB-EP742755EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Lipoyl synthase 1 (lipA1) CSB-YP742756EYP
CSB-EP742756EYP
CSB-BP742756EYP
CSB-MP742756EYP
CSB-EP742756EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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