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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Inactive rhomboid protein 2 (RHBDF2), partial CSB-YP744137HU
CSB-EP744137HU
CSB-BP744137HU
CSB-MP744137HU
CSB-EP744137HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein TMED8 (TMED8) CSB-YP744138HU
CSB-EP744138HU
CSB-BP744138HU
CSB-MP744138HU
CSB-EP744138HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP744139RNX
CSB-EP744139RNX
CSB-BP744139RNX
CSB-MP744139RNX
CSB-EP744139RNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc transporter 9 (SLC30A9), partial CSB-YP744140HU1
CSB-EP744140HU1
CSB-BP744140HU1
CSB-MP744140HU1
CSB-EP744140HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat B- and T-lymphocyte attenuator (Btla), partial CSB-YP744141RA
CSB-EP744141RA
CSB-BP744141RA
CSB-MP744141RA
CSB-EP744141RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Aquaporin-1 (AQP1), partial CSB-YP744142PI1
CSB-EP744142PI1
CSB-BP744142PI1
CSB-MP744142PI1
CSB-EP744142PI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Telomere-associated protein RIF1 (Rif1), partial CSB-YP744143MO
CSB-EP744143MO
CSB-BP744143MO
CSB-MP744143MO
CSB-EP744143MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Atlastin-1 (Atl1), partial CSB-YP744144RA1
CSB-EP744144RA1
CSB-BP744144RA1
CSB-MP744144RA1
CSB-EP744144RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Aminoacylase-1B (Acy1b) CSB-YP744145RA
CSB-EP744145RA
CSB-BP744145RA
CSB-MP744145RA
CSB-EP744145RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Smith-Magenis syndrome chromosomal region candidate gene 8-B protein homolog (smcr8b), partial CSB-YP744146DIL
CSB-EP744146DIL
CSB-BP744146DIL
CSB-MP744146DIL
CSB-EP744146DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Keratin, type I cytoskeletal 14 (KRT14) CSB-YP744147CH
CSB-EP744147CH
CSB-BP744147CH
CSB-MP744147CH
CSB-EP744147CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida milleri 3-isopropylmalate dehydrogenase (LEU2) CSB-YP744148CHAJ
CSB-EP744148CHAJ
CSB-BP744148CHAJ
CSB-MP744148CHAJ
CSB-EP744148CHAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calsyntenin-1 (Clstn1), partial CSB-YP744149RA
CSB-EP744149RA
CSB-BP744149RA
CSB-MP744149RA
CSB-EP744149RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human coronavirus NL63 Nucleoprotein (N) CSB-YP744150HIX
CSB-MP744150HIX
CSB-EP744150HIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polistes dominula Venom phospholipase A1 1 CSB-YP744151POG
CSB-EP744151POG
CSB-BP744151POG
CSB-MP744151POG
CSB-EP744151POG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant European mountain ash ringspot-associated virus Envelope glycoprotein, partial CSB-YP744152EAAM1
CSB-EP744152EAAM1
CSB-BP744152EAAM1
CSB-MP744152EAAM1
CSB-EP744152EAAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep 40S ribosomal protein S26 (RPS26) CSB-YP744153SH
CSB-EP744153SH
CSB-BP744153SH
CSB-MP744153SH
CSB-EP744153SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-like modifier HUB1 (HUB1) CSB-YP744154SVG
CSB-EP744154SVG
CSB-BP744154SVG
CSB-MP744154SVG
CSB-EP744154SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YPR053C (YPR053C) CSB-YP744155SVG
CSB-EP744155SVG
CSB-BP744155SVG
CSB-MP744155SVG
CSB-EP744155SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit 14-3-3 protein theta (YWHAQ) CSB-YP744156RB
CSB-EP744156RB
CSB-BP744156RB
CSB-MP744156RB
CSB-EP744156RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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