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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lachancea kluyveri Uracil catabolism protein 2 (URC2), partial CSB-YP744338LMA
CSB-EP744338LMA
CSB-BP744338LMA
CSB-MP744338LMA
CSB-EP744338LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyto alba Aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL), partial CSB-YP744339TCAG
CSB-EP744339TCAG
CSB-BP744339TCAG
CSB-MP744339TCAG
CSB-EP744339TCAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis niloticus Doublesex- and mab-3-related transcription factor A2 (dmrta2) CSB-YP744340OET
CSB-EP744340OET
CSB-BP744340OET
CSB-MP744340OET
CSB-EP744340OET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein E (sev)2B (drk) CSB-YP744341DMJ
CSB-EP744341DMJ
CSB-BP744341DMJ
CSB-MP744341DMJ
CSB-EP744341DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio 60S ribosomal protein L41 (rpl41) CSB-YP744342EQE
CSB-EP744342EQE
CSB-BP744342EQE
CSB-MP744342EQE
CSB-EP744342EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Phospholipase A2, beta bungarotoxin A3 chain CSB-YP744343BPN
CSB-EP744343BPN
CSB-BP744343BPN
CSB-MP744343BPN
CSB-EP744343BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-tRNA hydrolase (pth) CSB-YP744344FKZ
CSB-EP744344FKZ
CSB-BP744344FKZ
CSB-MP744344FKZ
CSB-EP744344FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Acyl carrier protein (acpP) CSB-YP744345OBN
CSB-EP744345OBN
CSB-BP744345OBN
CSB-MP744345OBN
CSB-EP744345OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP744346OBN
CSB-EP744346OBN
CSB-BP744346OBN
CSB-MP744346OBN
CSB-EP744346OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP744347OBN
CSB-EP744347OBN
CSB-BP744347OBN
CSB-MP744347OBN
CSB-EP744347OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP744348OBN
CSB-EP744348OBN
CSB-BP744348OBN
CSB-MP744348OBN
CSB-EP744348OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Putative Holliday junction resolvase (PAM_304) CSB-YP744349OBN
CSB-EP744349OBN
CSB-BP744349OBN
CSB-MP744349OBN
CSB-EP744349OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Glucose-6-phosphate isomerase (pgi) CSB-YP744350OBN
CSB-EP744350OBN
CSB-BP744350OBN
CSB-MP744350OBN
CSB-EP744350OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP744351OBN
CSB-EP744351OBN
CSB-BP744351OBN
CSB-MP744351OBN
CSB-EP744351OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP744352OBN
CSB-EP744352OBN
CSB-BP744352OBN
CSB-MP744352OBN
CSB-EP744352OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S5 (rpsE) CSB-YP744353OBN
CSB-EP744353OBN
CSB-BP744353OBN
CSB-MP744353OBN
CSB-EP744353OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S14 (rpsN) CSB-YP744354OBN
CSB-EP744354OBN
CSB-BP744354OBN
CSB-MP744354OBN
CSB-EP744354OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S19 (rpsS) CSB-YP744355OBN
CSB-EP744355OBN
CSB-BP744355OBN
CSB-MP744355OBN
CSB-EP744355OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S2 (rpsB) CSB-YP744356OBN
CSB-EP744356OBN
CSB-BP744356OBN
CSB-MP744356OBN
CSB-EP744356OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Glutamate--tRNA ligase (gltX) CSB-YP744357OBN
CSB-EP744357OBN
CSB-BP744357OBN
CSB-MP744357OBN
CSB-EP744357OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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