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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus UPF0736 protein BCE_1296 (BCE_1296) CSB-YP744738BQN
CSB-EP744738BQN
CSB-BP744738BQN
CSB-MP744738BQN
CSB-EP744738BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3'-5' exoribonuclease yhaM (yhaM) CSB-YP744739BQN
CSB-EP744739BQN
CSB-BP744739BQN
CSB-MP744739BQN
CSB-EP744739BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP744740BQN
CSB-EP744740BQN
CSB-BP744740BQN
CSB-MP744740BQN
CSB-EP744740BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0295 protein BCE_0593 (BCE_0593), partial CSB-YP744741BQN1
CSB-EP744741BQN1
CSB-BP744741BQN1
CSB-MP744741BQN1
CSB-EP744741BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Proline--tRNA ligase 2 (proS2) CSB-YP744742BQN
CSB-EP744742BQN
CSB-BP744742BQN
CSB-MP744742BQN
CSB-EP744742BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized RNA methyltransferase BCE_0363 (BCE_0363) CSB-YP744743BQN
CSB-EP744743BQN
CSB-BP744743BQN
CSB-MP744743BQN
CSB-EP744743BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP744744BQN
CSB-EP744744BQN
CSB-BP744744BQN
CSB-MP744744BQN
CSB-EP744744BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine import ATP-binding protein MetN 1 (metN1) CSB-YP744745BQN
CSB-EP744745BQN
CSB-BP744745BQN
CSB-MP744745BQN
CSB-EP744745BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP744746BQN
CSB-EP744746BQN
CSB-BP744746BQN
CSB-MP744746BQN
CSB-EP744746BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S13 (rpsM) CSB-YP744747BQN
CSB-EP744747BQN
CSB-BP744747BQN
CSB-MP744747BQN
CSB-EP744747BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L18 (rplR) CSB-YP744748BQN
CSB-EP744748BQN
CSB-BP744748BQN
CSB-MP744748BQN
CSB-EP744748BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L14 (rplN) CSB-YP744749BQN
CSB-EP744749BQN
CSB-BP744749BQN
CSB-MP744749BQN
CSB-EP744749BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S10 (rpsJ) CSB-YP744750BQN
CSB-EP744750BQN
CSB-BP744750BQN
CSB-MP744750BQN
CSB-EP744750BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Type III pantothenate kinase (coaX) CSB-YP744751BQN
CSB-EP744751BQN
CSB-BP744751BQN
CSB-MP744751BQN
CSB-EP744751BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP744752BQN
CSB-EP744752BQN
CSB-BP744752BQN
CSB-MP744752BQN
CSB-EP744752BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP744753BQN
CSB-EP744753BQN
CSB-BP744753BQN
CSB-MP744753BQN
CSB-EP744753BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP744754WAQ
CSB-EP744754WAQ
CSB-BP744754WAQ
CSB-MP744754WAQ
CSB-EP744754WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase gamma chain (atpG) CSB-YP744755WAQ
CSB-EP744755WAQ
CSB-BP744755WAQ
CSB-MP744755WAQ
CSB-EP744755WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S20 (rpsT) CSB-YP744756WAQ
CSB-EP744756WAQ
CSB-BP744756WAQ
CSB-MP744756WAQ
CSB-EP744756WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative 3-methyladenine DNA glycosylase (WD_1110) CSB-YP744757WAQ
CSB-EP744757WAQ
CSB-BP744757WAQ
CSB-MP744757WAQ
CSB-EP744757WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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