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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Homoserine kinase (thrB) CSB-YP746102EYO
CSB-EP746102EYO
CSB-BP746102EYO
CSB-MP746102EYO
CSB-EP746102EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable inorganic polyphosphate/ATP-NAD kinase 1 (ppnK1) CSB-YP746103EYO
CSB-EP746103EYO
CSB-BP746103EYO
CSB-MP746103EYO
CSB-EP746103EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP746104EYO
CSB-EP746104EYO
CSB-BP746104EYO
CSB-MP746104EYO
CSB-EP746104EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE), partial CSB-YP746105EYO
CSB-EP746105EYO
CSB-BP746105EYO
CSB-MP746105EYO
CSB-EP746105EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I reaction center subunit IV (psaE) CSB-YP746106EYO
CSB-EP746106EYO
CSB-BP746106EYO
CSB-MP746106EYO
CSB-EP746106EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Shikimate kinase (aroK) CSB-YP746107EYO
CSB-EP746107EYO
CSB-BP746107EYO
CSB-MP746107EYO
CSB-EP746107EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Putative imidazole glycerol phosphate synthase subunit hisF2 (hisF2) CSB-YP746108EYO
CSB-EP746108EYO
CSB-BP746108EYO
CSB-MP746108EYO
CSB-EP746108EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L35 (rpmI) CSB-YP746109EYO
CSB-EP746109EYO
CSB-BP746109EYO
CSB-MP746109EYO
CSB-EP746109EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein grpE (grpE) CSB-YP746110EYO
CSB-EP746110EYO
CSB-BP746110EYO
CSB-MP746110EYO
CSB-EP746110EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 7-carboxy-7-deazaguanine synthase (queE) CSB-YP746111EYP
CSB-EP746111EYP
CSB-BP746111EYP
CSB-MP746111EYP
CSB-EP746111EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L9 (rplI) CSB-YP746112EYP
CSB-EP746112EYP
CSB-BP746112EYP
CSB-MP746112EYP
CSB-EP746112EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Trigger factor (tig) CSB-YP746113EYP
CSB-EP746113EYP
CSB-BP746113EYP
CSB-MP746113EYP
CSB-EP746113EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein CrcB homolog 2 (crcB2), partial CSB-YP746114EYP1
CSB-EP746114EYP1
CSB-BP746114EYP1
CSB-MP746114EYP1
CSB-EP746114EYP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I iron-sulfur center (psaC) CSB-YP746115EYP
CSB-EP746115EYP
CSB-BP746115EYP
CSB-MP746115EYP
CSB-EP746115EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L3 (rplC) CSB-YP746116EYP
CSB-EP746116EYP
CSB-BP746116EYP
CSB-MP746116EYP
CSB-EP746116EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L29 (rpmC) CSB-YP746117EYP
CSB-EP746117EYP
CSB-BP746117EYP
CSB-MP746117EYP
CSB-EP746117EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L18 (rplR) CSB-YP746118EYP
CSB-EP746118EYP
CSB-BP746118EYP
CSB-MP746118EYP
CSB-EP746118EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L17 (rplQ) CSB-YP746119EYP
CSB-EP746119EYP
CSB-BP746119EYP
CSB-MP746119EYP
CSB-EP746119EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S7 (rpsG) CSB-YP746120EYP
CSB-EP746120EYP
CSB-BP746120EYP
CSB-MP746120EYP
CSB-EP746120EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribose-5-phosphate isomerase A (rpiA) CSB-YP746121EYP
CSB-EP746121EYP
CSB-BP746121EYP
CSB-MP746121EYP
CSB-EP746121EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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