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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chromobacterium violaceum Succinylglutamate desuccinylase (astE) CSB-YP745702CKA
CSB-EP745702CKA
CSB-BP745702CKA
CSB-MP745702CKA
CSB-EP745702CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Tryptophan synthase beta chain (trpB) CSB-YP745703CKA
CSB-EP745703CKA
CSB-BP745703CKA
CSB-MP745703CKA
CSB-EP745703CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP745704CKA1
CSB-EP745704CKA1
CSB-BP745704CKA1
CSB-MP745704CKA1
CSB-EP745704CKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Diaminopimelate epimerase (dapF) CSB-YP745705CKA
CSB-EP745705CKA
CSB-BP745705CKA
CSB-MP745705CKA
CSB-EP745705CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Tyrosine recombinase XerC (xerC) CSB-YP745706CKA
CSB-EP745706CKA
CSB-BP745706CKA
CSB-MP745706CKA
CSB-EP745706CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP745707CKA
CSB-EP745707CKA
CSB-BP745707CKA
CSB-MP745707CKA
CSB-EP745707CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribosome-recycling factor (frr) CSB-YP745708CKA
CSB-EP745708CKA
CSB-BP745708CKA
CSB-MP745708CKA
CSB-EP745708CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP745709CKA
CSB-EP745709CKA
CSB-BP745709CKA
CSB-MP745709CKA
CSB-EP745709CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0234 protein CV_2047 (CV_2047) CSB-YP745710CKA
CSB-EP745710CKA
CSB-BP745710CKA
CSB-MP745710CKA
CSB-EP745710CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Chaperone protein ClpB (clpB), partial CSB-YP745711CKA
CSB-EP745711CKA
CSB-BP745711CKA
CSB-MP745711CKA
CSB-EP745711CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Cyanate hydratase (cynS) CSB-YP745712CKA
CSB-EP745712CKA
CSB-BP745712CKA
CSB-MP745712CKA
CSB-EP745712CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Dihydrodipicolinate reductase (dapB) CSB-YP745713CKA
CSB-EP745713CKA
CSB-BP745713CKA
CSB-MP745713CKA
CSB-EP745713CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum L-threonine 3-dehydrogenase (tdh) CSB-YP745714CKA
CSB-EP745714CKA
CSB-BP745714CKA
CSB-MP745714CKA
CSB-EP745714CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Pantothenate synthetase (panC) CSB-YP745715CKA
CSB-EP745715CKA
CSB-BP745715CKA
CSB-MP745715CKA
CSB-EP745715CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Regulatory protein recX (recX) CSB-YP745716CKA
CSB-EP745716CKA
CSB-BP745716CKA
CSB-MP745716CKA
CSB-EP745716CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP745717CKA
CSB-EP745717CKA
CSB-BP745717CKA
CSB-MP745717CKA
CSB-EP745717CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribosome maturation factor RimP (rimP) CSB-YP745718CKA
CSB-EP745718CKA
CSB-BP745718CKA
CSB-MP745718CKA
CSB-EP745718CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP745719CKA
CSB-EP745719CKA
CSB-BP745719CKA
CSB-MP745719CKA
CSB-EP745719CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Chaperone protein htpG (htpG), partial CSB-YP745720CKA
CSB-EP745720CKA
CSB-BP745720CKA
CSB-MP745720CKA
CSB-EP745720CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UPF0246 protein CV_1250 (CV_1250) CSB-YP745721CKA
CSB-EP745721CKA
CSB-BP745721CKA
CSB-MP745721CKA
CSB-EP745721CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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