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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter hepaticus Tryptophan--tRNA ligase (trpS) CSB-YP746182HCO
CSB-EP746182HCO
CSB-BP746182HCO
CSB-MP746182HCO
CSB-EP746182HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP746183HCO
CSB-EP746183HCO
CSB-BP746183HCO
CSB-MP746183HCO
CSB-EP746183HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Leucine--tRNA ligase (leuS), partial CSB-YP746184HCO
CSB-EP746184HCO
CSB-BP746184HCO
CSB-MP746184HCO
CSB-EP746184HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Protein TolB (tolB) CSB-YP746185HCO
CSB-EP746185HCO
CSB-BP746185HCO
CSB-MP746185HCO
CSB-EP746185HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Glutamate 5-kinase (proB) CSB-YP746186HCO
CSB-EP746186HCO
CSB-BP746186HCO
CSB-MP746186HCO
CSB-EP746186HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 30S ribosomal protein S12 (rpsL) CSB-YP746187HCO
CSB-EP746187HCO
CSB-BP746187HCO
CSB-MP746187HCO
CSB-EP746187HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Phosphoglycerate kinase (pgk) CSB-YP746188HCO
CSB-EP746188HCO
CSB-BP746188HCO
CSB-MP746188HCO
CSB-EP746188HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP746189HCO
CSB-EP746189HCO
CSB-BP746189HCO
CSB-MP746189HCO
CSB-EP746189HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP746190HCO
CSB-EP746190HCO
CSB-BP746190HCO
CSB-MP746190HCO
CSB-EP746190HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Carbon storage regulator homolog (csrA) CSB-YP746191HCO
CSB-EP746191HCO
CSB-BP746191HCO
CSB-MP746191HCO
CSB-EP746191HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Elongation factor 4 (lepA), partial CSB-YP746192HCO
CSB-EP746192HCO
CSB-BP746192HCO
CSB-MP746192HCO
CSB-EP746192HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus UvrABC system protein C (uvrC), partial CSB-YP746193HCO
CSB-EP746193HCO
CSB-BP746193HCO
CSB-MP746193HCO
CSB-EP746193HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi DNA mismatch repair protein MutS (mutS), partial CSB-YP746194HSY
CSB-EP746194HSY
CSB-BP746194HSY
CSB-MP746194HSY
CSB-EP746194HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Probable Fe (2+)-trafficking protein (HD_2003) CSB-YP746195HSY
CSB-EP746195HSY
CSB-BP746195HSY
CSB-MP746195HSY
CSB-EP746195HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 50S ribosomal protein L16 (rplP) CSB-YP746196HSY
CSB-EP746196HSY
CSB-BP746196HSY
CSB-MP746196HSY
CSB-EP746196HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 50S ribosomal protein L6 (rplF) CSB-YP746197HSY
CSB-EP746197HSY
CSB-BP746197HSY
CSB-MP746197HSY
CSB-EP746197HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 30S ribosomal protein S11 (rpsK) CSB-YP746198HSY
CSB-EP746198HSY
CSB-BP746198HSY
CSB-MP746198HSY
CSB-EP746198HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP746199HSY
CSB-EP746199HSY
CSB-BP746199HSY
CSB-MP746199HSY
CSB-EP746199HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP746200HSY
CSB-EP746200HSY
CSB-BP746200HSY
CSB-MP746200HSY
CSB-EP746200HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP746201HSY
CSB-EP746201HSY
CSB-BP746201HSY
CSB-MP746201HSY
CSB-EP746201HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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