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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Haemophilus ducreyi Macrodomain Ter protein (matP) CSB-YP746242HSY
CSB-EP746242HSY
CSB-BP746242HSY
CSB-MP746242HSY
CSB-EP746242HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP746243HSY
CSB-EP746243HSY
CSB-BP746243HSY
CSB-MP746243HSY
CSB-EP746243HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi Ribosomal protein L11 methyltransferase (prmA) CSB-YP746244HSY
CSB-EP746244HSY
CSB-BP746244HSY
CSB-MP746244HSY
CSB-EP746244HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi ATP synthase subunit b (atpF), partial CSB-YP746245HSY
CSB-EP746245HSY
CSB-BP746245HSY
CSB-MP746245HSY
CSB-EP746245HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Queuine tRNA-ribosyltransferase (tgt) CSB-YP746246BHM
CSB-EP746246BHM
CSB-BP746246BHM
CSB-MP746246BHM
CSB-EP746246BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP746247BHM
CSB-EP746247BHM
CSB-BP746247BHM
CSB-MP746247BHM
CSB-EP746247BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S8 (rpsH) CSB-YP746248BHM
CSB-EP746248BHM
CSB-BP746248BHM
CSB-MP746248BHM
CSB-EP746248BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L2 (rplB) CSB-YP746249BHM
CSB-EP746249BHM
CSB-BP746249BHM
CSB-MP746249BHM
CSB-EP746249BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19 (rplS) CSB-YP746250BHM
CSB-EP746250BHM
CSB-BP746250BHM
CSB-MP746250BHM
CSB-EP746250BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP746251BHM
CSB-EP746251BHM
CSB-BP746251BHM
CSB-MP746251BHM
CSB-EP746251BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP746252BHM
CSB-EP746252BHM
CSB-BP746252BHM
CSB-MP746252BHM
CSB-EP746252BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein signal peptidase (lspA), partial CSB-YP746253BHM1
CSB-EP746253BHM1
CSB-BP746253BHM1
CSB-MP746253BHM1
CSB-EP746253BHM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-binding factor A (rbfA) CSB-YP746254BHM
CSB-EP746254BHM
CSB-BP746254BHM
CSB-MP746254BHM
CSB-EP746254BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S6 (rpsF) CSB-YP746255BHM
CSB-EP746255BHM
CSB-BP746255BHM
CSB-MP746255BHM
CSB-EP746255BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein HldE (hldE) CSB-YP746256BHM
CSB-EP746256BHM
CSB-BP746256BHM
CSB-MP746256BHM
CSB-EP746256BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP746257BHM
CSB-EP746257BHM
CSB-BP746257BHM
CSB-MP746257BHM
CSB-EP746257BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit alpha (atpA), partial CSB-YP746258BHM
CSB-EP746258BHM
CSB-BP746258BHM
CSB-MP746258BHM
CSB-EP746258BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP746259BHM
CSB-EP746259BHM
CSB-BP746259BHM
CSB-MP746259BHM
CSB-EP746259BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate--tRNA ligase (aspS), partial CSB-YP746260BHM
CSB-EP746260BHM
CSB-BP746260BHM
CSB-MP746260BHM
CSB-EP746260BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase beta chain (trpB) CSB-YP746261BHM
CSB-EP746261BHM
CSB-BP746261BHM
CSB-MP746261BHM
CSB-EP746261BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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