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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Translation initiation factor IF-1 (infA) CSB-YP746262BHM
CSB-EP746262BHM
CSB-BP746262BHM
CSB-MP746262BHM
CSB-EP746262BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP746263BHM1
CSB-EP746263BHM1
CSB-BP746263BHM1
CSB-MP746263BHM1
CSB-EP746263BHM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP746264BHM
CSB-EP746264BHM
CSB-BP746264BHM
CSB-MP746264BHM
CSB-EP746264BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-3 (infC) CSB-YP746265BHM
CSB-EP746265BHM
CSB-BP746265BHM
CSB-MP746265BHM
CSB-EP746265BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apolipoprotein N-acyltransferase (lnt), partial CSB-YP746266BHM
CSB-EP746266BHM
CSB-BP746266BHM
CSB-MP746266BHM
CSB-EP746266BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate dehydratase (aroQ) CSB-YP746267BHM
CSB-EP746267BHM
CSB-BP746267BHM
CSB-MP746267BHM
CSB-EP746267BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-3-O- (3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD) CSB-YP746268BHM
CSB-EP746268BHM
CSB-BP746268BHM
CSB-MP746268BHM
CSB-EP746268BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP746269BHM
CSB-EP746269BHM
CSB-BP746269BHM
CSB-MP746269BHM
CSB-EP746269BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP746270BHM
CSB-EP746270BHM
CSB-BP746270BHM
CSB-MP746270BHM
CSB-EP746270BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N utilization substance protein B homolog (nusB) CSB-YP746271BHM
CSB-EP746271BHM
CSB-BP746271BHM
CSB-MP746271BHM
CSB-EP746271BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L28 (rpmB) CSB-YP746272BHM
CSB-EP746272BHM
CSB-BP746272BHM
CSB-MP746272BHM
CSB-EP746272BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP746273BHM
CSB-EP746273BHM
CSB-BP746273BHM
CSB-MP746273BHM
CSB-EP746273BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP746274BHM
CSB-EP746274BHM
CSB-BP746274BHM
CSB-MP746274BHM
CSB-EP746274BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP746275BHM
CSB-EP746275BHM
CSB-BP746275BHM
CSB-MP746275BHM
CSB-EP746275BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP746276BHM
CSB-EP746276BHM
CSB-BP746276BHM
CSB-MP746276BHM
CSB-EP746276BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease subunit alpha (ureC), partial CSB-YP746277BHM
CSB-EP746277BHM
CSB-BP746277BHM
CSB-MP746277BHM
CSB-EP746277BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP746278BHM
CSB-EP746278BHM
CSB-BP746278BHM
CSB-MP746278BHM
CSB-EP746278BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP746279BHM
CSB-EP746279BHM
CSB-BP746279BHM
CSB-MP746279BHM
CSB-EP746279BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Protein-export protein SecB (secB) CSB-YP746280BUA
CSB-EP746280BUA
CSB-BP746280BUA
CSB-MP746280BUA
CSB-EP746280BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Ornithine aminotransferase (rocD) CSB-YP746281BUA
CSB-EP746281BUA
CSB-BP746281BUA
CSB-MP746281BUA
CSB-EP746281BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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