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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella bronchiseptica Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP746442BTY
CSB-EP746442BTY
CSB-BP746442BTY
CSB-MP746442BTY
CSB-EP746442BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Adenosylhomocysteinase (ahcY) CSB-YP746443BTY
CSB-EP746443BTY
CSB-BP746443BTY
CSB-MP746443BTY
CSB-EP746443BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Malate synthase G (glcB), partial CSB-YP746444BTY
CSB-EP746444BTY
CSB-BP746444BTY
CSB-MP746444BTY
CSB-EP746444BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 30S ribosomal protein S13 (rpsM) CSB-YP746445BTY
CSB-EP746445BTY
CSB-BP746445BTY
CSB-MP746445BTY
CSB-EP746445BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 50S ribosomal protein L15 (rplO) CSB-YP746446BTY
CSB-EP746446BTY
CSB-BP746446BTY
CSB-MP746446BTY
CSB-EP746446BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DNA-directed RNA polymerase II subunit RPB9 (RPB9) CSB-YP746447CZI
CSB-EP746447CZI
CSB-BP746447CZI
CSB-MP746447CZI
CSB-EP746447CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Spindle assembly checkpoint component MAD1 (MAD1), partial CSB-YP746448CZI
CSB-EP746448CZI
CSB-BP746448CZI
CSB-MP746448CZI
CSB-EP746448CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 21S rRNA pseudouridine (2819) synthase (PUS5) CSB-YP746449CZI
CSB-EP746449CZI
CSB-BP746449CZI
CSB-MP746449CZI
CSB-EP746449CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein CFT1 (CFT1), partial CSB-YP746450CZI
CSB-EP746450CZI
CSB-BP746450CZI
CSB-MP746450CZI
CSB-EP746450CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Spindle pole component BBP1 (BBP1) CSB-YP746451CZI
CSB-EP746451CZI
CSB-BP746451CZI
CSB-MP746451CZI
CSB-EP746451CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Cytochrome b-c1 complex subunit 2, mitochondrial (QCR2) CSB-YP746452CZI
CSB-EP746452CZI
CSB-BP746452CZI
CSB-MP746452CZI
CSB-EP746452CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata COP9 signalosome complex subunit 5 (RRI1) CSB-YP746453CZI
CSB-EP746453CZI
CSB-BP746453CZI
CSB-MP746453CZI
CSB-EP746453CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Nonsense-mediated decay protein 4 (NMD4) CSB-YP746454CZI
CSB-EP746454CZI
CSB-BP746454CZI
CSB-MP746454CZI
CSB-EP746454CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) CSB-YP746455CZI
CSB-EP746455CZI
CSB-BP746455CZI
CSB-MP746455CZI
CSB-EP746455CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial thiamine pyrophosphate carrier 1 (TPC1), partial CSB-YP746456CZI1
CSB-EP746456CZI1
CSB-BP746456CZI1
CSB-MP746456CZI1
CSB-EP746456CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DASH complex subunit DAD2 (DAD2) CSB-YP746457CZI
CSB-EP746457CZI
CSB-BP746457CZI
CSB-MP746457CZI
CSB-EP746457CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ribosome-recycling factor, mitochondrial (RRF1), partial CSB-YP746458CZI
CSB-EP746458CZI
CSB-BP746458CZI
CSB-MP746458CZI
CSB-EP746458CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Nuclear fusion protein KAR5 (KAR5), partial CSB-YP746459CZI
CSB-EP746459CZI
CSB-BP746459CZI
CSB-MP746459CZI
CSB-EP746459CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata RuvB-like helicase 1 (RVB1) CSB-YP746460CZI
CSB-EP746460CZI
CSB-BP746460CZI
CSB-MP746460CZI
CSB-EP746460CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ubiquinone biosynthesis protein COQ4, mitochondrial (COQ4) CSB-YP746461CZI
CSB-EP746461CZI
CSB-BP746461CZI
CSB-MP746461CZI
CSB-EP746461CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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