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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida glabrata GMP synthase [glutamine-hydrolyzing] (GUA1), partial CSB-YP746462CZI
CSB-EP746462CZI
CSB-BP746462CZI
CSB-MP746462CZI
CSB-EP746462CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Serine hydroxymethyltransferase, cytosolic (SHM2) CSB-YP746463CZI
CSB-EP746463CZI
CSB-BP746463CZI
CSB-MP746463CZI
CSB-EP746463CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Nucleolar protein 9 (NOP9), partial CSB-YP746464CZI
CSB-EP746464CZI
CSB-BP746464CZI
CSB-MP746464CZI
CSB-EP746464CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (LYS5) CSB-YP746465CZI
CSB-EP746465CZI
CSB-BP746465CZI
CSB-MP746465CZI
CSB-EP746465CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein OS-9 homolog (YOS9), partial CSB-YP746466CZI
CSB-EP746466CZI
CSB-BP746466CZI
CSB-MP746466CZI
CSB-EP746466CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ras modification protein ERF4 (ERF4) CSB-YP746467CZI
CSB-EP746467CZI
CSB-BP746467CZI
CSB-MP746467CZI
CSB-EP746467CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DNA mismatch repair protein MSH3 (MSH3), partial CSB-YP746468CZI
CSB-EP746468CZI
CSB-BP746468CZI
CSB-MP746468CZI
CSB-EP746468CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP746469CZI
CSB-EP746469CZI
CSB-BP746469CZI
CSB-MP746469CZI
CSB-EP746469CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 78 kDa glucose-regulated protein homolog (KAR2), partial CSB-YP746470CZI
CSB-EP746470CZI
CSB-BP746470CZI
CSB-MP746470CZI
CSB-EP746470CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pre-rRNA-processing protein RIX1 (RIX1), partial CSB-YP746471CZI
CSB-EP746471CZI
CSB-BP746471CZI
CSB-MP746471CZI
CSB-EP746471CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Acyl-protein thioesterase 1 (CAGL0D02398g) CSB-YP746472CZI
CSB-EP746472CZI
CSB-BP746472CZI
CSB-MP746472CZI
CSB-EP746472CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mediator of RNA polymerase II transcription subunit 1 (MED1), partial CSB-YP746473CZI
CSB-EP746473CZI
CSB-BP746473CZI
CSB-MP746473CZI
CSB-EP746473CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DNA repair protein RAD59 (RAD59) CSB-YP746474CZI
CSB-EP746474CZI
CSB-BP746474CZI
CSB-MP746474CZI
CSB-EP746474CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata NAD-dependent histone deacetylase SIR2 (SIR2), partial CSB-YP746475CZI
CSB-EP746475CZI
CSB-BP746475CZI
CSB-MP746475CZI
CSB-EP746475CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ubiquitin-related modifier 1 (URM1) CSB-YP746476CZI
CSB-EP746476CZI
CSB-BP746476CZI
CSB-MP746476CZI
CSB-EP746476CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Nuclear distribution protein PAC1 (PAC1) CSB-YP746477CZI
CSB-EP746477CZI
CSB-BP746477CZI
CSB-MP746477CZI
CSB-EP746477CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein PBN1 (PBN1), partial CSB-YP746478CZI
CSB-EP746478CZI
CSB-BP746478CZI
CSB-MP746478CZI
CSB-EP746478CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DNA polymerase epsilon subunit D (DPB4) CSB-YP746479CZI
CSB-EP746479CZI
CSB-BP746479CZI
CSB-MP746479CZI
CSB-EP746479CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DNA polymerase epsilon subunit C (DPB3) CSB-YP746480CZI
CSB-EP746480CZI
CSB-BP746480CZI
CSB-MP746480CZI
CSB-EP746480CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata GPI mannosyltransferase 1 (GPI14), partial CSB-YP746481CZI
CSB-EP746481CZI
CSB-BP746481CZI
CSB-MP746481CZI
CSB-EP746481CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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