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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis Protein MAK16 homolog (mak16) CSB-YP747424XBF
CSB-EP747424XBF
CSB-BP747424XBF
CSB-MP747424XBF
CSB-EP747424XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Basic leucine zipper and W2 domain-containing protein 1 (Bzw1) CSB-YP747425RA
CSB-EP747425RA
CSB-BP747425RA
CSB-MP747425RA
CSB-EP747425RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis DDB1- and CUL4-associated factor 12 (dcaf12) CSB-YP747426XBF
CSB-EP747426XBF
CSB-BP747426XBF
CSB-MP747426XBF
CSB-EP747426XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Glycerol-3-phosphate dehydrogenase 1-like protein (gpd1l) CSB-YP747427XBF
CSB-EP747427XBF
CSB-BP747427XBF
CSB-MP747427XBF
CSB-EP747427XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Glycine amidinotransferase, mitochondrial (gatm) CSB-YP747428XBF
CSB-EP747428XBF
CSB-BP747428XBF
CSB-MP747428XBF
CSB-EP747428XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/threonine-protein phosphatase 4 catalytic subunit (ppp4c) CSB-YP747429XBF
CSB-EP747429XBF
CSB-BP747429XBF
CSB-MP747429XBF
CSB-EP747429XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Eukaryotic translation initiation factor 3 subunit L (eif3l), partial CSB-YP747430XBF
CSB-EP747430XBF
CSB-BP747430XBF
CSB-MP747430XBF
CSB-EP747430XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis H/ACA ribonucleoprotein complex subunit 2-like protein (nhp2) CSB-YP747431XBF
CSB-EP747431XBF
CSB-BP747431XBF
CSB-MP747431XBF
CSB-EP747431XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Interleukin enhancer-binding factor 2 homolog (ilf2) CSB-YP747432XBF
CSB-EP747432XBF
CSB-BP747432XBF
CSB-MP747432XBF
CSB-EP747432XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytochrome c oxidase assembly protein COX11, mitochondrial (Cox11), partial CSB-YP747433MO
CSB-EP747433MO
CSB-BP747433MO
CSB-MP747433MO
CSB-EP747433MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein B-Myc (Mycb) CSB-YP747434MO
CSB-EP747434MO
CSB-BP747434MO
CSB-MP747434MO
CSB-EP747434MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Palmitoleoyl-protein carboxylesterase NOTUM (NOTUM) CSB-YP747435HU
CSB-BP747435HU
CSB-MP747435HU
CSB-EP747435HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative polypeptide N-acetylgalactosaminyltransferase-like protein 4 (GALNTL4), partial CSB-YP747436HU
CSB-EP747436HU
CSB-BP747436HU
CSB-MP747436HU
CSB-EP747436HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Coiled-coil domain-containing protein 62 (CCDC62) CSB-YP747437HU
CSB-EP747437HU
CSB-BP747437HU
CSB-MP747437HU
CSB-EP747437HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Structural maintenance of chromosomes protein 6 (smc6), partial CSB-YP747438XBE
CSB-EP747438XBE
CSB-BP747438XBE
CSB-MP747438XBE
CSB-EP747438XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Kinesin-like protein KIF11 (Kif11), partial CSB-YP747439MO
CSB-EP747439MO
CSB-BP747439MO
CSB-MP747439MO
CSB-EP747439MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ATP-dependent RNA helicase DDX51 (Ddx51) CSB-YP747440MO
CSB-EP747440MO
CSB-BP747440MO
CSB-MP747440MO
CSB-EP747440MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat COMM domain-containing protein 3 (Commd3) CSB-YP747441RA
CSB-EP747441RA
CSB-BP747441RA
CSB-MP747441RA
CSB-EP747441RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Prostate androgen-regulated mucin-like protein 1 homolog (Parm1), partial CSB-YP747442RA
CSB-EP747442RA
CSB-BP747442RA
CSB-MP747442RA
CSB-EP747442RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (Smarcd3) CSB-YP747443MO
CSB-EP747443MO
CSB-BP747443MO
CSB-MP747443MO
CSB-EP747443MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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