Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant L-ectoine synthase (ectC) CSB-YP746382BIK
CSB-EP746382BIK
CSB-BP746382BIK
CSB-MP746382BIK
CSB-EP746382BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP746383BIK
CSB-EP746383BIK
CSB-BP746383BIK
CSB-MP746383BIK
CSB-EP746383BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP746384BIK
CSB-EP746384BIK
CSB-BP746384BIK
CSB-MP746384BIK
CSB-EP746384BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucine--tRNA ligase (leuS), partial CSB-YP746385BIK
CSB-EP746385BIK
CSB-BP746385BIK
CSB-MP746385BIK
CSB-EP746385BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19 (rplS) CSB-YP746386BIK
CSB-EP746386BIK
CSB-BP746386BIK
CSB-MP746386BIK
CSB-EP746386BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP746387BIK
CSB-EP746387BIK
CSB-BP746387BIK
CSB-MP746387BIK
CSB-EP746387BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Ts (tsf) CSB-YP746388BIK
CSB-EP746388BIK
CSB-BP746388BIK
CSB-MP746388BIK
CSB-EP746388BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable chorismate--pyruvate lyase (ubiC) CSB-YP746389BIK
CSB-EP746389BIK
CSB-BP746389BIK
CSB-MP746389BIK
CSB-EP746389BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglycerate kinase (pgk) CSB-YP746390BIK
CSB-EP746390BIK
CSB-BP746390BIK
CSB-MP746390BIK
CSB-EP746390BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0234 protein BPP1084 (BPP1084) CSB-YP746391BIK
CSB-EP746391BIK
CSB-BP746391BIK
CSB-MP746391BIK
CSB-EP746391BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative 5' (3')-deoxyribonucleotidase (BPP0431) CSB-YP746392BIK
CSB-EP746392BIK
CSB-BP746392BIK
CSB-MP746392BIK
CSB-EP746392BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ribonuclease H (rnhA) CSB-YP746393BTY
CSB-EP746393BTY
CSB-BP746393BTY
CSB-MP746393BTY
CSB-EP746393BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP746394BTY
CSB-EP746394BTY
CSB-BP746394BTY
CSB-MP746394BTY
CSB-EP746394BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Probable parvulin-type peptidyl-prolyl cis-trans isomerase (BB3803) CSB-YP746395BTY
CSB-EP746395BTY
CSB-BP746395BTY
CSB-MP746395BTY
CSB-EP746395BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ribonuclease 3 (rnc) CSB-YP746396BTY
CSB-EP746396BTY
CSB-BP746396BTY
CSB-MP746396BTY
CSB-EP746396BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica tRNA modification GTPase MnmE (mnmE) CSB-YP746397BTY
CSB-EP746397BTY
CSB-BP746397BTY
CSB-MP746397BTY
CSB-EP746397BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP746398BTY
CSB-EP746398BTY
CSB-BP746398BTY
CSB-MP746398BTY
CSB-EP746398BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Type IV secretion system protein ptlI homolog (ptlI) CSB-YP746399BTY
CSB-EP746399BTY
CSB-BP746399BTY
CSB-MP746399BTY
CSB-EP746399BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Large-conductance mechanosensitive channel (mscL), partial CSB-YP746400BTY1
CSB-EP746400BTY1
CSB-BP746400BTY1
CSB-MP746400BTY1
CSB-EP746400BTY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Histidinol-phosphate aminotransferase 2 (hisC2) CSB-YP746401BTY
CSB-EP746401BTY
CSB-BP746401BTY
CSB-MP746401BTY
CSB-EP746401BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>