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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Proteim yippee-like 3 (ypel3) CSB-YP747364DIL
CSB-EP747364DIL
CSB-BP747364DIL
CSB-MP747364DIL
CSB-EP747364DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Intraflagellar transport protein 122 homolog (Ift122), partial CSB-YP747365MO
CSB-EP747365MO
CSB-BP747365MO
CSB-MP747365MO
CSB-EP747365MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein FAM188A (fam188a) CSB-YP747366XBF
CSB-EP747366XBF
CSB-BP747366XBF
CSB-MP747366XBF
CSB-EP747366XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Armadillo repeat-containing protein 6 (ARMC6) CSB-YP747367HU
CSB-EP747367HU
CSB-BP747367HU
CSB-MP747367HU
CSB-EP747367HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type II cytoskeletal 73 (Krt73) CSB-YP747368MO
CSB-EP747368MO
CSB-BP747368MO
CSB-MP747368MO
CSB-EP747368MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse UPF0485 protein C1orf144 homolog (D4Ertd22e) CSB-YP747369MO
CSB-EP747369MO
CSB-BP747369MO
CSB-MP747369MO
CSB-EP747369MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADP-dependent oxidoreductase domain-containing protein 1 (NOXRED1) CSB-YP747370HU
CSB-EP747370HU
CSB-BP747370HU
CSB-MP747370HU
CSB-EP747370HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone H3.3C (H3F3C) CSB-YP747371HU
CSB-EP747371HU
CSB-BP747371HU
CSB-MP747371HU
CSB-EP747371HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DNA-directed RNA polymerase III subunit RPC7 (Polr3g) CSB-YP747372MO
CSB-EP747372MO
CSB-BP747372MO
CSB-MP747372MO
CSB-EP747372MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial (zgc:77082) CSB-YP747373DIL
CSB-EP747373DIL
CSB-BP747373DIL
CSB-MP747373DIL
CSB-EP747373DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phostensin (PPP1R18) CSB-YP747374HU
CSB-EP747374HU
CSB-BP747374HU
CSB-MP747374HU
CSB-EP747374HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Dihydropyrimidine dehydrogenase [NADP (+)] (dpyd), partial CSB-YP747375DIL
CSB-EP747375DIL
CSB-BP747375DIL
CSB-MP747375DIL
CSB-EP747375DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Sprouty-related, EVH1 domain-containing protein 2 (spred2) CSB-YP747376DIL
CSB-EP747376DIL
CSB-BP747376DIL
CSB-MP747376DIL
CSB-EP747376DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Solute carrier family 22 member 6 (slc22a6), partial CSB-YP747377DIL
CSB-EP747377DIL
CSB-BP747377DIL
CSB-MP747377DIL
CSB-EP747377DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Immediate early response gene 5-like protein (ier5l) CSB-YP747378DIL
CSB-EP747378DIL
CSB-BP747378DIL
CSB-MP747378DIL
CSB-EP747378DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Histone chaperone asf1b-A (asf1ba) CSB-YP747379DIL
CSB-EP747379DIL
CSB-BP747379DIL
CSB-MP747379DIL
CSB-EP747379DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Calcium-binding mitochondrial carrier protein SCaMC-2-A (slc25a25a), partial CSB-YP747380DIL1
CSB-EP747380DIL1
CSB-BP747380DIL1
CSB-MP747380DIL1
CSB-EP747380DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein arginine N-methyltransferase 6 (Prmt6) CSB-YP747381MO
CSB-EP747381MO
CSB-BP747381MO
CSB-MP747381MO
CSB-EP747381MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Signal peptide, CUB and EGF-like domain-containing protein 1 (Scube1), partial CSB-YP747382MO
CSB-EP747382MO
CSB-BP747382MO
CSB-MP747382MO
CSB-EP747382MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio ADP-ribosylation factor-like protein 8B-A (arl8ba) CSB-YP747383DIL
CSB-EP747383DIL
CSB-BP747383DIL
CSB-MP747383DIL
CSB-EP747383DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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