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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Desulfovibrio vulgaris Dihydroxy-acid dehydratase (ilvD), partial CSB-YP747964DDH
CSB-EP747964DDH
CSB-BP747964DDH
CSB-MP747964DDH
CSB-EP747964DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP747965DDH
CSB-EP747965DDH
CSB-BP747965DDH
CSB-MP747965DDH
CSB-EP747965DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Carbamoyl-phosphate synthase small chain (carA) CSB-YP747966DDH
CSB-EP747966DDH
CSB-BP747966DDH
CSB-MP747966DDH
CSB-EP747966DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Phosphate acetyltransferase (pta), partial CSB-YP747967DDH
CSB-EP747967DDH
CSB-BP747967DDH
CSB-MP747967DDH
CSB-EP747967DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP747968DDH
CSB-EP747968DDH
CSB-BP747968DDH
CSB-MP747968DDH
CSB-EP747968DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L31 (rpmE) CSB-YP747969DDH
CSB-EP747969DDH
CSB-BP747969DDH
CSB-MP747969DDH
CSB-EP747969DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Putative nickel-responsive regulator (DVU_2891) CSB-YP747970DDH
CSB-EP747970DDH
CSB-BP747970DDH
CSB-MP747970DDH
CSB-EP747970DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris FMN-dependent NADH-azoreductase (azoR) CSB-YP747971DDH
CSB-EP747971DDH
CSB-BP747971DDH
CSB-MP747971DDH
CSB-EP747971DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L13 (rplM) CSB-YP747972DDH
CSB-EP747972DDH
CSB-BP747972DDH
CSB-MP747972DDH
CSB-EP747972DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Nucleoside diphosphate kinase (ndk) CSB-YP747973DDH
CSB-EP747973DDH
CSB-BP747973DDH
CSB-MP747973DDH
CSB-EP747973DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP747974DDH
CSB-EP747974DDH
CSB-BP747974DDH
CSB-MP747974DDH
CSB-EP747974DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Thymidylate kinase (tmk) CSB-YP747975DDH
CSB-EP747975DDH
CSB-BP747975DDH
CSB-MP747975DDH
CSB-EP747975DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Lipoprotein signal peptidase (lspA), partial CSB-YP747976DDH1
CSB-EP747976DDH1
CSB-BP747976DDH1
CSB-MP747976DDH1
CSB-EP747976DDH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP747977DDH
CSB-EP747977DDH
CSB-BP747977DDH
CSB-MP747977DDH
CSB-EP747977DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP747978DDH
CSB-EP747978DDH
CSB-BP747978DDH
CSB-MP747978DDH
CSB-EP747978DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP747979DDH
CSB-EP747979DDH
CSB-BP747979DDH
CSB-MP747979DDH
CSB-EP747979DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP747980DDH
CSB-EP747980DDH
CSB-BP747980DDH
CSB-MP747980DDH
CSB-EP747980DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Triosephosphate isomerase (tpiA) CSB-YP747981DDH
CSB-EP747981DDH
CSB-BP747981DDH
CSB-MP747981DDH
CSB-EP747981DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Protein CrcB homolog (crcB), partial CSB-YP747982DDH1
CSB-EP747982DDH1
CSB-BP747982DDH1
CSB-MP747982DDH1
CSB-EP747982DDH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Glutamyl-tRNA reductase (hemA) CSB-YP747983DDH
CSB-EP747983DDH
CSB-BP747983DDH
CSB-MP747983DDH
CSB-EP747983DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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